FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4884731
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC65620.13433697781924941No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG6100.041.5916949
CGAAAGC28300.032.2772833
AGCGAAA28350.032.0039561
GCGAAAG31050.029.435462
TAGGGTA19350.026.597265
GGGTACT19600.026.2585477
TAATACA59100.025.5127724
GATGCGC8900.025.2487628
TTATTCG4455.456968E-1222.807901145
ATACAGC52200.022.7741266
ATAATAC55050.022.255753
TATAATA60850.021.9340652
CTAGGGT25800.021.9157664
GCTAGGG24450.021.6449643
GTCCTAT40850.020.9670181
TACAGCC52700.020.082177
GCCGACC4702.382876E-1020.052237145
TCTATAC58500.019.9518363
CTATACA38600.019.9067484
TACACGT8800.019.7701644
Produced by FastQC (version 0.11.5)