FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCTTACAGTCTTCCCCCCTTATATGATCATGATAAACCTGGTCGCCC12.0408163265306123No Hit
CCCCAGCTCGCCCCTGTGCAAACATTAGAACACTGTATTTTTAAATCTGC12.0408163265306123No Hit
GGCTCTGAAGAAACAGATTATACCCAGTTAGGTTGGAGGAAATGGGAAAA12.0408163265306123No Hit
CCTTTAATGGTGGCATATATGTTGGAAAGAGAATTGGTTCGCAACACCAG12.0408163265306123No Hit
AAAAAGCGTCCAAAGACAACACCTACACTCGGAGCCCAGCTCTGTACTCA12.0408163265306123No Hit
CCAACAAGCAGGTGACAGAAACCTACAGCAAGCTGGCAATCAACAACTCC12.0408163265306123No Hit
AAATAAAGTAGAGAATAATAACCAACATAAAGTATAAATCTGATCAACAC12.0408163265306123No Hit
TTTTAGGGGAGTAATTGCCATCATACGCTTACTTGAAGGTCATTTCAGGA12.0408163265306123No Hit
TAAGTACCGTGTGGAGAGCTCGCTCAGATTGATACGCTCAGATTGGTCCC12.0408163265306123No Hit
GTGCTATATACTAAGCATCAACTGAAATACGTAAAATAAACATGAGACAT12.0408163265306123No Hit
TCTTGTAATTGATTTCTATTTATATACAACTGTGCTTGGAAAAGAACGTA12.0408163265306123No Hit
CAAAAGGCACTACTACTCAACAACAAATTAAAAAGCACTCACAATTCAGT12.0408163265306123No Hit
ACACTAATCTCATGTAATCATTATTTTCTTTTTAACATTTATTATCAAAA12.0408163265306123No Hit
GCTTCACTTAGCTTACATCATATATGCTCTACATATATAGTAGCTACAAT12.0408163265306123No Hit
AAGCAGGACTGTTTTGCAAAAGCATAAACGTCCAGTTTCACAGAAGTGGG12.0408163265306123No Hit
TCCCTGGTTAACATGAGACATGACAATAAATGTTCCACACGTGATAATTC12.0408163265306123No Hit
AAGTATGATAGTTCTAGGACCAACTCTGTTTTATTAAACATAACACCTCG12.0408163265306123No Hit
ATCACACATAAATTGGATGTCTCGATAAGTGCACAGCTCCCCTCATAAAA12.0408163265306123No Hit
CTCTCTTCCACACCGCCCCTTAAGCAACTACTCATACTCCCCTCCCCTGC12.0408163265306123No Hit
CCTCTGTCTATATCCGGCAGCAACACAGTCTTGCTGACATCAAGCTTTTC12.0408163265306123No Hit
GTCCAGCGCTCTCTCACACGAGCTTGATTTCTTGATTTGAGCCAAAAACA12.0408163265306123No Hit
GGGTACCACATTTTAGAAACTGTTTTACTGAATTCTGTGAATTTTGTTTC12.0408163265306123No Hit
GCGTTTATTCATCTTGTAATTCTGTCAGAGTTGCCCAAGGAATGGTATTC12.0408163265306123No Hit
ACTTGATTAGTAAGTTGAGCTCTGTGACTCCTGATCTTGTGGTTTATAGT12.0408163265306123No Hit
ACCAAAGTGAAATCAGTAGATTTGAAAACTAAGACCTGAAAACATTAATA12.0408163265306123No Hit
GTGTACAAAAACTCTCAAATGACAATAAAAAGTTACTTAAAAGTCATATT12.0408163265306123No Hit
GCATAAAAAAGTACAATCAAACACAAACACAGGCAAACACTCACACCTGC12.0408163265306123No Hit
CTAAACTTCTACCTGCATTGCTGGCCTAGAGAATATGGACAACCAGAGAG12.0408163265306123No Hit
CATCATAACTTAAATAAATTAAAAACAAACTAAAAATACAATAGAAAGAT12.0408163265306123No Hit
TCTCCGGTAACTGTAAAGGAGAGCTCTGTGTCTTGTGAGTTAGTCATCAT12.0408163265306123No Hit
TCCCCGTGGTGATGTGATCCCTGACTTGACAGGTGCTGCAGCACTGAAGG12.0408163265306123No Hit
ATTAATGACAGTAAATGGAAACACAAAAAGTTGCACTGACCAAAGTTGTA12.0408163265306123No Hit
ATGAAAGTAAGCCCTCAAAAATCTTGTAAAAAAACTTATTCTGGGGCTAC12.0408163265306123No Hit
CTTTTGAATAATCCTTCTTCCATCCAGGAAGCAAAACTCCTCATAAAAAA12.0408163265306123No Hit
CTATGCGAATCCTATCCTCAGTCCGAAAGAGGCGAAGAGACAATTGAAGA12.0408163265306123No Hit
TTCAATTGACTTTTTAGTTAGTATTTCATTAATTTAAGTTCAGATGTGTT12.0408163265306123No Hit
CATTACATGCGCCTGCCAGCAAATCCATCGTGGGTTTTTTTTTTTTTTTA12.0408163265306123No Hit
GGGGAGAGCAAAGAGGAGAGAGGAATGGAGTCAGACAGTGAGGTGGGGGG12.0408163265306123No Hit
GCCAATGCCATTGCCTCAGGTCATGATCCCAGAGCCCTCGGATCCAGCCC12.0408163265306123No Hit
ATTATGAGCTGTCTCTTACACTAGCATTCTGTGGACTTGCAGACATTTTA12.0408163265306123No Hit
CGATGGAAAGATAACCTACTCCTTCACAATTTAGTGATGGAACAATTCAC12.0408163265306123No Hit
GCCCCACCTCGCCCCGCTGGACACACCCGGAACCACCCCACTGACCATAA12.0408163265306123No Hit
TACAAAAACAGTATAAATTCCTTATCTGAAATTAAAATATTATATATTCT12.0408163265306123No Hit
NTATGTATCAGTTTGCTTCTCTCTTCAAAAATATAGCAAAAAGACATAGT12.0408163265306123No Hit
ATATTGAGGTAATTGCATCAAATCATTAAAATCAGATGAAATGGTTAAAT12.0408163265306123No Hit
GGTTGAAGCTTACTTGGAATGGCGGAATCCAATGCATTAATGGCAAAGAG12.0408163265306123No Hit
CTTGGGCAAGTCAATTAACCTCTCTGAAACTCTGTTTCCATATCTCTTAA12.0408163265306123No Hit
ACCACATACTCCATCTAACAAAAAACAAGGGACAAGACACACGAATATAC12.0408163265306123No Hit
GGCTCACAAATTATAAATAACATAAGCAGAAATGTAAAGAATTAAAACGA12.0408163265306123No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)