FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4646972
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC7400.033.3580741
CTACGCA8800.028.0272452
TATACTG28100.027.8598675
GTTACGT4950.023.4675941
CGAAAGC19900.022.587373
GTATTAT54700.022.4312321
GTTGACA34850.022.0829371
TATACCG1650.002604281521.966784
GTTATGT34950.021.3965531
GTAGCAT53950.020.9935991
GCGAAAG21950.020.1594872
ACATGGT38200.019.5449925
AGCGAAA22950.019.2974951
CGCACTG15200.019.0755925
TAGCATG51400.018.7703652
AGGTCGG19350.018.3561428
GTAATAT19550.018.1971381
CTACTGA48400.018.1218136
TACTGAA54550.017.540837
TATAATA48450.017.0684952
Produced by FastQC (version 0.11.5)