FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGGACGGATAAGCAAGAGTAGGCTGGAGCAAACGCGCCAGCGTCGC15.0No Hit
CCTAAGCCCAGTCTGGGTTCCTACCCACCCGAGGTCTACCTTCCCTCCAC15.0No Hit
TAGTAGGTGCTCAAGAAATGCTAGGTGTTCTGATTCCTGGACCTACCCTG15.0No Hit
TTCCATGACCAGTGCAGAGCCCAAGGTGGAGTTTGATCTCACAACCCTGA15.0No Hit
GAATAAGCCGCACCTGCCGTGCCAGCCCAGGCTGGAGAAGACGCGCCAGC15.0No Hit
GGTTTATGTTGTTAAGTGGGCAGCATATGGATGCGCTGTTTATCGATCCT15.0No Hit
CCAATGGTGGGTACGAAACCTAAAGTTCAGCCCACCGGGATGAGAAAAAA15.0No Hit
CATTTATTCCGCACCAACGCAAAAAAGCCCAGTCTTCCGACTGGGCTCTT15.0No Hit
CTCTTCCTTCCTATTCAGTTAGGTTCGGCAGGGGCTGTGGTCCTGTTTAT15.0No Hit
GCCAGATCCTACTGACGGTGATGCATCTGCTAGAGCTCCTTAGAGATCAA15.0No Hit
ACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCC15.0No Hit
CTATATTCAATCAGTTAGATCACTCACTCAGGATTTCCCAGCACCAAAGT15.0No Hit
TAATTACGAAAAAGACATGATTGTCTAATCACTCTCTCACTGAGGTTACT15.0No Hit
TTAGTAGAAACAAGGCTGGCTCACATGCTCTTAAACGTTAATGGTCTATA15.0No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT15.0No Hit
TCTAAGCACGAAACTTGCCCCGACATGAGTTCTCCCTGACTCCTTGAGGG15.0No Hit
ATGCTTAGTCTGAGCTTGGTTATCTTACCTGCCTAGACACAAATGGAGTG15.0No Hit
CTTCACCTTTATCGTAGCTCAGACTTAGCCCCACACCGTCGGCACGTACG15.0No Hit
CACATGTCTGTGTCACACACGTTGAGGCACACATTTATACCCGAAACACC15.0No Hit
GAAGTGTCTGTGAAAGAGTGTGTACCTGAATTGAATGAGTGTTGGGCTCA15.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)