FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6169700
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG8500.024.7332679
TATACTG28150.024.4637435
CGAAAGC32650.022.2051853
GCGAAAG33350.022.185042
AGCGAAA33900.022.0532051
TGATCCG7050.020.565699
CTATACG2556.971506E-519.899524
GTCTACG4752.6921043E-1019.8647881
ACTACGC5703.0649971E-916.5539911
GTCCTAT52350.016.3606111
CCTATAC16900.016.301763
GTATTAT52500.015.2078411
ATACCGC18850.014.9979186
TATATAC36250.014.3999853
TACACCG11700.014.2501585
ATTCGCG4602.6863869E-514.1836199
TATAATA49750.014.1427722
CTATACT21600.014.0954944
GTATGAC15950.014.0908943
ACCGTGC17600.014.0043068
Produced by FastQC (version 0.11.5)