FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4646407
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC7750.040.2758871
TATACTG22950.030.3207635
TGATCCG2808.22547E-925.8891019
CTACGCA13000.023.9920082
GCGAAAG22350.023.366652
AGCGAAA23050.022.989541
CGCACTG14550.022.418225
GTAATAT18700.021.738261
TACGCAC16300.020.0145873
CGAAAGC25950.019.5561083
GTTACGT5204.3655746E-1119.543531
GTTGACA31650.019.2656611
TAGTGCA32900.019.168959
CTAATAC24450.018.6802833
GTATAAG13050.018.3561571
GGTAGTG33350.018.257637
AGGTCGG16800.018.1219798
ACGCACT18450.018.0736474
GCCGACC5751.8371793E-1017.651323145
GTATTAT47150.016.6272491
Produced by FastQC (version 0.11.5)