FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4909710
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC65650.13371461858236025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC6100.036.84105145
GCGAAAG16600.034.0809972
AGCGAAA22800.024.5099721
CGAAAGC24600.023.2809623
TATACTG27650.022.8080865
GCGTTAC3808.956704E-921.0085471
ACTACGC3951.39862095E-820.2107541
GTCCTAT49250.019.8936521
TCTATAC70200.019.3113863
TGACGCG2307.972678E-418.911394145
GTATAGG11050.018.3900411
TATACCG4003.5750054E-718.1228669
CTATACC72550.018.0848434
GTGATCG9050.017.6222329
GTTCTAT75400.017.229341
TATACCA77600.017.187825
GTTGACA35700.017.0764661
TACACCG10250.016.9727465
CGTTACA4307.5833486E-716.8677562
CTACGCA5602.4647306E-916.827864
Produced by FastQC (version 0.11.5)