FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1802_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1802_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5720536
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC243200.42513498735083566No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT127260.22246167142379666No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA82290.14385015669860307No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG81410.14231183931016253No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA80430.14059871312758104No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA75260.131561098470493No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG72700.12708599334048418No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT71850.12560011859028594No Hit
ATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATTTG69980.12233119413984983No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT68160.11914967408648422No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA65570.11462212631823313No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA64580.11289151925623753No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC58800.10278757095488955No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG57410.10035772871633009No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACA51350.048.3545231
ACATGGT56350.043.4789435
GACATGG59250.041.4750184
GCAGTGC40000.039.9313621
CCGTATA12500.038.85574145
CAGTGCA41700.038.617532
TTGACAT64400.038.296642
GGTCCTA85700.036.539859
GTGATCG8350.036.4608359
GCGTTAA2900.034.9940229
TGACATG73500.034.1273
GCGAAAG9050.033.6642042
AGTGCAG49000.033.140673
TGGTCCT96250.032.5344168
ATGGTCC95850.032.5186547
CATGGTC75350.032.4192436
GTTCTGA60850.031.7374821
GTGCAGC52100.031.0271844
GCTACTA15350.029.7506149
GTTCTAT144400.029.7147241
Produced by FastQC (version 0.11.5)