FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1801_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629195
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG2151.2732926E-1137.111992
GTGATCG4400.034.5980269
GCGAAAG12100.032.3719182
AGCGAAA13750.028.5128381
TAGCGGT2901.1945303E-824.9965884
CGAAAGC16200.024.6145693
TGATCCG2951.43245416E-824.5732549
ATAGCGG3052.0381776E-823.7708473
TATACTG23200.023.1212065
GTATTAT45150.020.5826641
AGCGGTA3601.1802331E-720.1355865
TAGTGCA29150.019.3972749
TATTATC28800.019.393592
GCGGTAT4202.8681825E-818.984986
ATAGACG4253.2783646E-818.7649883
GCCGACC5601.2551027E-1018.124273145
TAAGGTA29750.018.031154
GTCCTAT31200.017.4524361
ACTGTGC32600.017.1218938
TATACAG21400.016.9364745
Produced by FastQC (version 0.11.5)