FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1801_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2948858
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT63880.2166262329349192No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC49930.16931978413338317No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG34750.1178422290934321No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC32820.11129732255673212No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC30000.10173429849792699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT4050.050.1832471
TTACGTG4950.043.955982
GTTGACA21350.038.078151
ACGTGTT6650.034.8832244
ACATGGT23100.034.203145
GACGACT2800.033.656864
TAGCATG33900.031.449922
TATACTG15650.031.0321565
AGCGAAA12550.029.4972931
GACATGG27100.029.4246754
GCGAAAG13250.029.0109462
GTGATCG2752.1464075E-1028.9947139
GCATGTT37850.028.1539754
TGACATG30250.028.040493
CTACTGA33600.027.8292756
TACGTGT8050.027.0178323
CGAAAGC14000.026.9277733
GTTATGT21800.026.6372411
AGCATGT41300.025.6287783
ACGACTC3409.094947E-1125.5831365
Produced by FastQC (version 0.11.5)