FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1801_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589405
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT139790.30459286116609885No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC127190.2771383218521791No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC69460.15134859529721173No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT67520.14712146781554472No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC67110.14622810582199652No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC57980.1263344594778626No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG57170.12456952480768203No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA53060.11561411555528439No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA51510.11223677143333394No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT51290.11175740646118615No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC47520.10354283398392601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT4800.040.8401871
TTACGTG5450.037.266972
TATACTG19300.037.1836245
TTAGGTA24000.030.20424
ATTAGGT25950.026.8191853
AGCGAAA16150.026.0747811
GTTGACA25300.025.8277871
GGATTAG30450.025.036131
GTATTAT47450.024.3291031
GTAATAT18250.024.2678991
CGACGAA1500.001495426424.16573145
GCGAAAG17500.024.0409982
ACCGTAC1851.8142149E-423.5474971
TAGGTAG32100.023.4856265
GATTAGG29800.023.3677852
AGCACCG99900.023.3649165
ACCGTGT101150.022.861688
CGAAAGC18550.022.6670913
GCACCGT101550.022.6283656
ACGTGTT8750.022.3683664
Produced by FastQC (version 0.11.5)