FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1801_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923693
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC134810.2737985491784317No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT116100.23579861701369279No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC81540.16560740078636096No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT79670.161809438565727No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA73050.1483642461055147No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC72520.14728781831036175No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT58750.11932100559478424No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC58640.119097596052394No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA58050.11789930850684639No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC57910.11761496908925882No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG54910.11152198156952516No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA53200.10804897868327695No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT50280.1021184708307362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG24100.034.589985
GCTATAT7550.033.6583181
GTAATAT19300.031.6004521
AGCGAAA19150.029.573121
CGACGAA1804.7065714E-628.193146145
GCGAAAG21450.027.7306792
TAAGGTA38650.026.2579774
GTTGACA27450.026.1856981
TTAGGTA27200.026.1184
TAGCATG38450.025.8462872
CGAAAGC23300.025.5141073
TAGTGCA41550.025.4713139
GGTAGTG40950.024.7824157
TAGTCGG4151.8189894E-1224.4537248
ATTAGGT27950.024.3819523
ATAAGGT44150.023.8100193
TAATCGT2451.9172512E-623.6697359
GATAGCG3402.5120244E-923.454803145
AGCACCG86650.023.340235
ACCGTGT90250.022.8105748
Produced by FastQC (version 0.11.5)