FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1801_d04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGTTCTAAGAGAAGAGAGAGAGAAGCACATTGCTCTTCTGGTAATA22.8169014084507045No Hit
GCATATACTTGGAACATCTCCACGTACAGAACATATGAGACCCAGATCCG11.4084507042253522No Hit
GTACCCTTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA11.4084507042253522No Hit
GATAATGACAGCTGACCATTTTCGGGGTCATGTACATTGCCTTAAACTTA11.4084507042253522No Hit
AGTCAGTGGAGCTCTGTGACTCCTGATCTTGTGGGTTTGAGTTTCAACCG11.4084507042253522No Hit
CAAATGTTCCCTAAGTTAGTTCATCTTTCTCTAACACGCCTATCGAAAAT11.4084507042253522No Hit
ATACCATGTTGTGCATCTACACTGCATTTTTTCCCCATTCATCTGGCGCT11.4084507042253522No Hit
GATTGATCACCACCTTGGTGGTGTTTAGTCACTCAATGTTTATTCCCTCG11.4084507042253522No Hit
CATTTTACAGTTGAGAAAGCCAAGGCTCTGAGAAGCTAAGTCACTTGCCC11.4084507042253522No Hit
CACTGTGGCTTACTGAGGTGTTTACAGGTAAGGACTCAGAAAGTAAATGT11.4084507042253522No Hit
GCTCCATGCTGCTAAGCGCGACAAAGGCGGGCCGACAGCCACAGACCCTG11.4084507042253522No Hit
CATGATTCCAAGGTCCTGGGATAGAGTCATGAATCAGGCTCCTTGCTCAG11.4084507042253522No Hit
GAATTTAAAGGGTTTAGGATGTACATCTGCTCCCTGATGTGTTCTGTAAG11.4084507042253522No Hit
GAACATCCAGCACAGCCTTAGACAAAGGCCCCAGGCCTCCGCCTCTGCTG11.4084507042253522No Hit
GACTGAGCAAAGTGGAAGGAGCAGCAGGCAGTAGAAGAGAGAGAAAGGGG11.4084507042253522No Hit
GTCATGGGTTGAATTGTATCTCTTCCCTCCCCCCATAATTAGTATGTTGA11.4084507042253522No Hit
TCCCAGCACATCTAAGACGTTGTCAGCCCACTGGGAATCCTGCAGTGGTT11.4084507042253522No Hit
ATCAGGTTCATCTATTATTTATAGCAATCAAAACAAACATAAAATGGACA11.4084507042253522No Hit
GGAGAGAGGCAATCATAACTTCGATTTGGATCTCCTTGAATTCGGGGTGT11.4084507042253522No Hit
CTTAGAATAGTACCTATGAGAGTAAGCCCTCAAAAAATGTTGTAAAAAAT11.4084507042253522No Hit
GTAACCCCCTGCGCCCCGTCGCAGGGCAGTTCTAATGCAGGGCTCTTCCT11.4084507042253522No Hit
GTATAAATTCACAAGCGTATCGGTCTTGCTTGCGTGATTTTTTTTTGTCA11.4084507042253522No Hit
GCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGAGACCTG11.4084507042253522No Hit
GACCCCGGTCTGACAGACTGTTGTAGTTAAGGCGAATGAAGGCAAGGTTG11.4084507042253522No Hit
GGGGTTGGCTGAGGCTGATGTTTCCGTTGTGATCTTCTGTCTGGATGGGG11.4084507042253522No Hit
TCGAGATGCCGTGTGCACACCAGCTGGCATGGGATAAATGGCTGGTCACT11.4084507042253522No Hit
CAATAGGTACTGAGCTCTGAGAGCCGCCGAGGATTTCCCATGATTATGAA11.4084507042253522No Hit
CCACAGGACGTAGGAGGGGGTTACCTTTGGGGGCATTAAGGGAGGGAGAA11.4084507042253522No Hit
GCTGTTATCAATACATATTGATTGGAAAGTGGATGATTCACTAAGGAAAA11.4084507042253522No Hit
TCAAAAAGCAGCAAAAGGAATATAATCCTCTCAAGTGCCCCTACAACGTT11.4084507042253522No Hit
GTGTTCTCCTTGGCGGTGCTGGTTCTCACACGGGGGTGGAAATTGTTTTT11.4084507042253522No Hit
GATTAGATCATCTTGGTCTCATTGAATTGATAAAGCCACAAAAATGCCAA11.4084507042253522No Hit
CTTCATGCTCCCCACTCAAGGAATCCAAGTTAAAACAAACTGAAGTTTAA11.4084507042253522No Hit
GTAGTATTGCTAGTGGTACTGAGGTCACTTTTTAGTTGAGCTTGTTGGTA11.4084507042253522No Hit
GTCTCAGAACCCGCGACAAAGCCAAGAGTCAGCCCCTCAGCTCACTGAGC11.4084507042253522No Hit
GAATGAACCAACCACCGCTTAAATAACACTATATCACCGGATAAGATAAA11.4084507042253522No Hit
ACATTGAGGTGATCCTTCCTGAAAAAGAGCAAATTGTCCCTAAACCAGAA11.4084507042253522No Hit
GTCTAGTTACATTCTACAATATATAGTCACCCAAGTTTCCCAGCACCACT11.4084507042253522No Hit
CCGTTTTATTACTATTTGACATGAGGAAGTCTCTAGTTCATTCGCCATGT11.4084507042253522No Hit
CCAGAGTTGCTTTTATTCATGGATGGTCAACTCTTGCCCATTTCAATAGC11.4084507042253522No Hit
ATTGAAAGCAAACTTTAGTGTGATTTTTAATCGACTTGAAGCTCTGATAC11.4084507042253522No Hit
GCTGAGCAGAGAACCCTATGCGGGGCTTGATCCCAGGACCCTGGGATCAT11.4084507042253522No Hit
GGTGACCTTTGCCTGGAACCCTGGGTGGAAGAAAATAAGCCTCCCATTAG11.4084507042253522No Hit
CAATAGACACACACACACACACACCCACACACCCACAGAGATTAGTATCA11.4084507042253522No Hit
CCAGAGCCCAATGCTCTTTCTCAGCATCGTCCGCCTCGCTCGCCAATAAA11.4084507042253522No Hit
CTACATTTTTTAAAGCAACGGGGTCAGTTATGAATCACTTACTGCTGTGT11.4084507042253522No Hit
GTGCCCTTCATTGGCTCTTGTTACAGTGTTTGGATTTTTTCTTGTTCTGC11.4084507042253522No Hit
ACATTCTACTGAACACCCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC11.4084507042253522No Hit
GGCTTGGGATCTTGGGGACAGAGCGGAGAACCAAGCCTAGGGAGAGAACT11.4084507042253522No Hit
AAGTTCCGCGAATAGAAGAAAATAGATTAAAATATAGATTATACATTCAT11.4084507042253522No Hit
TCAACTATAAGCTCATCTATCTTATTTTTGTTCATATTCAGTGTTGTTTG11.4084507042253522No Hit
TTTTTAATATATATCTTTCTATTGTCTTTTTAGTTTGTATTTAATTTATT11.4084507042253522No Hit
CCCATAAATATTATTATAAGAATAAAATTCTCTAGAGTGAAAGTTCATTA11.4084507042253522No Hit
GTGTCTGCTTAGTTCGGCCTGGCAGGGTGAACCCTGGGCACGAACTTGGA11.4084507042253522No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC11.4084507042253522No Hit
GTTTAGATACTGTACCTAAAGAAACTCACACCATGTTGTGCATATAAAGA11.4084507042253522No Hit
TCCCTCTACTGGCTCCTCCCACTTCTCCTCATCTTGGCCTCAGTGGCCCA11.4084507042253522No Hit
GAGAGAGAGAGAGCAAAGAGAGAGTCACAAGCAGACTCCACTCCCAGCAC11.4084507042253522No Hit
GGGCTGGATTGGCTGATGGCCTCGGCCAGTGTCCGCGGAGGGCCTCCCGG11.4084507042253522No Hit
GTCACTAGCTGGAACAGCCAGACCTTGGCATGGAAGTCCCAGCAGATCTA11.4084507042253522No Hit
TTTTCTGAGATTCCTGCTAAGCCAGTTTAAGCTGTTGCTAAACAAGGAAC11.4084507042253522No Hit
CTCTGCACCCCCATTGGATACCGTGTGTTACTGCACAAGGGTATTATTGT11.4084507042253522No Hit
ATTTCATCTCTCTACTGGTCATTAGTCTGTTCAGCTGTCTTTTTGTTTAG11.4084507042253522No Hit
CCCTTAGGGCTGGGGCCCAGAGACAGCCTAAATGCAGGGGAGGGAACCGG11.4084507042253522No Hit
GTGTGGGGAGGGATTAGGAGAGAAAGGTGGCAGCAGGCCCCTCGAGGACT11.4084507042253522No Hit
TACAAAATGAAACCTATCAAATCTGTGATTTTTTAAGGTCTTCAACCTGT11.4084507042253522No Hit
TATTGTCATATTACCCATTGGTCCTATGTCCGTTTTCGTACATCGTTTCG11.4084507042253522No Hit
CAAACTGACAGACTGCGCAAAGTGGAAGTCGCAGCAGGCAGTAGAAGAGG11.4084507042253522No Hit
TGTACGTCCATTACAGGCAGTAATCACAGGATAAGGTTGCCTTCCAGGAC11.4084507042253522No Hit
GGCCTGGCTGGCTGTTACAACAGAAATTTATTTTCTCACAGTTCTAGAGG11.4084507042253522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACT50.0145.0145
AACCCGC50.0145.08
ACCCGCG50.0145.09
AGAACCC50.0145.06
GAACCCG50.0145.07
TCAGAAC50.0145.04
CTCAGAA50.0145.03
CAGAACC50.0145.05
TCTCAGA50.0145.02
GTCTCAG100.072.51
Produced by FastQC (version 0.11.5)