FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1801_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686172
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA201550.4300951821657421No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC161410.3444389151742616No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG124460.26558991005878574No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA123020.2625170394940689No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT95710.20423919565905815No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA95420.2036203536703305No Hit
ACCCCTTACTTGGATGGACTTGACAGATACACACAGAACCCTCCATCCAA94760.20221195466150194No Hit
ATCTTAGTAGGTTGTATGTTTCTGGAAATGTATCCTTTTCTTTTGGATTA88830.18955770296096686No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT83580.17835452902710358No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA81990.17496156777856212No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT79830.17035226193148695No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG72990.15575612674908218No Hit
CTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAAGATGGTTG70830.151146820902007No Hit
GTTGACATGGTCCTATGATGTTGATCCTTTGTTTTGTGAATGCGGCACAT70510.1504639607765144No Hit
GTTTTCTTCTTGGAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA69270.1478178777902305No Hit
GCAGTGCAGCTACTAAATATGTAGAGCATATATGAACAAACCTAAGAAAA66750.14244035430197613No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA62660.13371254832302357No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA62400.1331577244710608No Hit
CTTATATGGACTTGACAGATACACACAGAACCCTCCATCCAAAAGCAGCA61500.13123718036811283No Hit
GTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTGGAATTGA60930.1300208357695791No Hit
GTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG54320.11591550630237217No Hit
CCCAACTGGTTGTTCAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATT49960.10661153709253524No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC49210.1050110836734119No Hit
GGATCATTCCTTCCTCTTGTTTTTCTGGCAGAGTTGGAGAAGGATTGGTA48960.10447759920037078No Hit
GGAGAGTTCTGTGTGTATCTGTCAAGTCCATCCAAGTAAGGGGTTGTCTG47860.10213026751898992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCA66350.072.486992
GCTACTA20450.071.959629
GCAGTGC69600.068.9474641
AGTGCAG74050.064.909323
GTGCAGC76500.062.7283944
CAGCAAC52750.062.6634987
CAGCTAC23850.061.0913737
ACATGGT60000.060.1658945
GTTACGT5800.058.829611
GTTGACA62900.058.2863461
TGCAGCA58800.056.70915
AGCTACT27750.053.0280088
GACATGG69500.051.836924
TTACGTG6850.049.7731062
TACGTGT6900.049.3818473
GCAGCTA29700.049.0582246
GGTTATA14950.046.618271
TGACATG86750.042.787713
CATGGTC86950.041.517586
TAATCGT3000.041.0784239
Produced by FastQC (version 0.11.5)