FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1801_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1801_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33342
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC510.15296023034011158No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGT100.007075872144.893396
CCTAGGT100.007075872144.893392
TAGACAT100.007075872144.893395
GCGAAAG100.007075872144.893392
ACGGTGC100.007075872144.893398
GGTTAAT259.3339215E-486.936041
CATAGAC300.001924902172.446693
CAGAGTC300.001924902172.446695
TATGGAT350.00354662862.097174
AGTATGG350.00354662862.097172
TCATAAC350.00354662862.097171
GATGTGT450.0095859548.29786
GTATGGA450.0095859548.29783
ATTGCCA400.007867918.13345520-24
CCCCCCC550.00260931115.830321105-109
GGGGGGG7000.09.328583145
Produced by FastQC (version 0.11.5)