FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252674
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC7050.044.2724951
GCGAAAG18450.031.8440882
AGCGAAA17600.031.7565081
CGAAAGC19050.031.5860883
CTACGCA10050.031.0343462
CGCACTG13650.024.9597765
AACGCGC2351.3336903E-624.677777
TATACTG31600.024.0867215
GTTACGT6850.023.312391
GTGATCG8200.022.9851519
ACGCACT14250.022.383174
TACGCAC14600.022.344593
GTATTAT72550.021.7108571
CTACTGA53700.020.3836676
TTACGTG8050.019.822892
GGTAGTG40200.018.3932277
TAGTGCA40750.018.323049
GCGGACC4404.8854417E-818.122919
GTTGACA33700.018.0927281
GTAGCAT66550.017.9966051
Produced by FastQC (version 0.11.5)