FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGAGAGGGTCAGAGGGAGAGAGGGTATCCTCCAGCAGCATTCCCACTG13.571428571428571No Hit
CAGAAAAACATTTTGCAAAGACTTTACAATTGATAATCAATCGATGTGTT13.571428571428571No Hit
GTCATGGCTTATACGAAGACTAATTCCATTATAATGACATAAAAAAACTA13.571428571428571No Hit
CCCCGGTGTCACATAACCTTAGCTCAAGTGGATCAAGCACAGAGGGGCAG13.571428571428571No Hit
ATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGT13.571428571428571No Hit
CTAGAGACCTTAAATAAGAAATCAAAAGCATCCAACAAGCAAAAGTCCAG13.571428571428571No Hit
CACTTTGAATATGTAGTTGTATTATAACTGCCTTATGCTCCTTACATATC13.571428571428571No Hit
CATCATTGACGATGAGTAAGTAATTGTCAGTTTCTCTGTTCCCTGTGTTT13.571428571428571No Hit
GAGGAAATGGCAAAATCAGGGAGGTAGAATTATCTTCACAGAGAAATTGT13.571428571428571No Hit
GTACTGAATTCACATCATAATCAATGGCCAAAAGAAGTAGCAAGTACTCA13.571428571428571No Hit
AGAGTAAGGAAAGTGTGAGACAGGAAATGACAAAACATGCTGGGGATTTC13.571428571428571No Hit
AAAGCAGGGAGCTGGCCAAGAGCAGGTGGGCTCGGCTGGGCTGGGGCCTG13.571428571428571No Hit
GTGCTAAATTACGTGTTACTCTCCCGGACTCAGCCGTCAGATAGATCAGG13.571428571428571No Hit
TTTTACAAGCTTGCACTGGCTTCAGTTGATAACTGATTAAAGATGTAAGT13.571428571428571No Hit
TATCAATTGAATTAAAGATATATAAATATGGAAGAAAGGTCCAACTATCT13.571428571428571No Hit
CATTAAAATTGGAAACTAACCGCAAACACATTGTATATACATACTAAGGA13.571428571428571No Hit
TACGAATGCAGATTGGAACATCCTGAAATTCACAATAAGTGCGATCCTCA13.571428571428571No Hit
GAGACAGGTAGCTATGAACAGAGAGAATGGGAAGAAGCTGGGAAGGGAGA13.571428571428571No Hit
TCTTTGCACAGAATGAGTAATACCCTCAGAACAGCGGACTCCACTACACC13.571428571428571No Hit
GACAAGACTAATCACCTAATCGGGAGCCCCAATTTAGAACCTGTTTTACT13.571428571428571No Hit
CTATTAAGGGAAGAGGTGGGGTGAGGGTGTGTGGAGGATAAAGTGTAGTG13.571428571428571No Hit
CCTCTAGATCAACTTCTATTAATTGAATCGATAAATTAACAATCAAATTA13.571428571428571No Hit
AGACAAAGCAGGGGGTCTACCACCAACTTGGCCGAATTTGTTAGGACGTC13.571428571428571No Hit
GACCAGAGAGGGGTTGAAACTCAAAACCACAAGATAAGGACTAACAGAAC13.571428571428571No Hit
GAAAAATACAGCATGTAAAATAGTACATTGGCTTCATTTACTAAGCAATA13.571428571428571No Hit
CCACTGAACCTCCCTGCAAATCTGCTGCTTTGCCTCAGCTTTTACAGTGT13.571428571428571No Hit
GCTTTGCATTCTAGGATCAATTTAATAAGTATTGATTGGACGCCCATTCT13.571428571428571No Hit
CACTAGGAGAGTAGCTTGCCAAGCCTGCTCAAAGAGACAAAAATTCAAGC13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)