FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439565
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT67200.12353929036604948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC11900.046.3565941
CTACGCA13000.042.959242
TATACTG28150.039.910485
GTAATAT28500.030.5619661
TACGCAC20350.028.1436353
GTATTAT63900.027.9433921
CGCACTG21350.027.4992475
GTATACG5300.026.0209181
AGCGAAA25750.025.3693981
ATACTGT50500.022.9647796
TACAGCC44300.022.5794327
GCGAAAG29350.022.2404522
TTACCAC33300.021.770344145
GCCGACC7350.021.699259145
GTTGACA30750.021.480341
TATTATC43600.021.4591452
CGAAAGC31350.021.0436633
GTAGCAT62650.020.3909051
ACGCACT28550.020.311854
TAATATA47150.019.8434522
Produced by FastQC (version 0.11.5)