FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4510389
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT57380.12721740852063979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC12050.042.1728131
CGATACA2300.037.825294145
TATACTG22700.035.7658125
CTACGCA13900.035.4841962
GTAATAT23400.029.473341
TACGCAC17200.029.0827263
AGCGAAA21750.029.0389921
GTATTAT54300.028.7448621
CCGACTA5550.028.735274
CGCACTG18550.028.526925
GCGAAAG23000.027.121362
TATTATC34650.027.0039482
TTACCAC28850.025.883339145
GTTACGT4450.024.4710641
CGAAAGC26300.023.4302883
GTTGACA24950.022.986781
ACGATAA2953.8814142E-722.11818145
GTATACG4006.8030204E-1021.7792471
GTAGCAT43400.021.4112421
CCGATCA3055.3543954E-721.3910989
Produced by FastQC (version 0.11.5)