FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3150587
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG52740.16739737706021132No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC45140.14327488813989267No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA41090.13042014075472286No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA40260.12778571104368805No Hit
TCTACTAACTTTGGACTTTATTTGTTCTTTTCTGTTTCCTTGAGGTATCA38760.12302469349362516No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT37320.11845411664556478No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG34520.10956688388544739No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGT32720.10385366282537191No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG31510.10001310866832117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAA1152.4883775E-950.430424145
AAGGTCG6950.044.8493929
ACTACGC4350.043.3901941
GCTACTA9900.043.2005969
GTTGACA26400.040.422291
CGATTAT750.001484500838.66333145
ACATGGT27900.037.9333845
CTACGCA5500.035.6098752
TATACTG26900.034.7624475
GTTATGT19950.034.569111
CAGTATG14300.033.963449
GTATACG2154.802132E-1033.765181
GATATGT15150.033.542311
GACAGTA14050.033.0199627
TTGACAT32500.032.586592
TTGGACA15500.032.2698674
GCAGTGC26150.032.2028121
GTAGAAA20850.032.0074582
TAGCATG51650.031.8804862
CAGTGCA27200.030.668912
Produced by FastQC (version 0.11.5)