FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1800_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1800_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4534471
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC120230.265146695171278No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA80670.17790388338573562No Hit
GCAGTGCAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAA73950.16308407309253936No Hit
TTACTGCCCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA65930.14539733521286166No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG61040.13461327682986615No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT60900.13430453078209123No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT59760.1317904558216383No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA57990.1278870236461982No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG48600.10717898515615162No Hit
CATCACCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA48240.10638506674758755No Hit
AAGTTGGAGAACGTTCTAGGGGCACTTGAGAGGAGTATATTCCTTTTGCT47280.10426795099141664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC7800.048.381891
GCTACTA9500.047.3079539
GCAGTGC34550.046.00131
GTTGACA47750.045.747481
TATTGCG657.346524E-444.6065187
ACATGGT49700.043.7529145
CAGTGCA36650.043.3329472
AGTGCAG40150.039.541093
GCCGACC5950.037.769356145
CTACGCA10050.037.521952
TGCAGCA31700.036.1276745
GACATGG60500.035.7028664
GTGCAGC45050.035.397294
GACGACT3100.035.0729034
GTTACGT4350.035.0351561
TGACATG64650.034.5360153
TTGACAT63500.034.1464542
TTACGTG4550.033.4700242
TACCCAC42850.032.662892
GGTTATA15400.032.51641
Produced by FastQC (version 0.11.5)