FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1797_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3715067
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA64090.1725137124041101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG21000.041.766715
GAGCTAT11900.036.549398
ACACCTA41350.033.8826751
GTAGCAT37350.031.4862061
ATCGAAT4900.031.068655145
AGCGAAA9450.030.727281
TTACGTG4600.029.9550742
CACCTAA49700.029.9138362
TAGCATG34000.029.6490332
AGCTATC14950.029.0949
GTTGACA22950.028.1516081
GTTACGT4900.028.1483821
TATCCGT2853.237801E-1027.979584
CCGACTA7000.027.9614124
CTACTGA41200.027.4467876
GCGAAAG11400.027.3550952
GTTATGT24500.025.777991
CGAAAGC12350.025.2406623
GTAATAT23650.024.8627831
ACATGGT26150.024.3935575
Produced by FastQC (version 0.11.5)