FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1797_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCATTGGCACATCTGAAAAATTAAGCAATATATAATTAGAAAAT21.9801980198019802No Hit
GAATTAAAACATTGCAACATTCAATGTGAAATAGATCGTCAACCAACCCC10.9900990099009901No Hit
GTCTGAGGGTGATGTTTCCTTTCTGATCTTCTGTCTGGATGGGGGATCAT10.9900990099009901No Hit
AGGTTGTAGTCCCGTATTGTGGCGGGCTGAGGTAGGAGATGGCGTGGGGG10.9900990099009901No Hit
AGCGAGTAGTGTGTCCCCACATTCCATAGCACCACAATCTATTGATCACA10.9900990099009901No Hit
CTTAAATAGACTCTATTTAATGCATGAGCAGAAGAAAAGCATTAGAACCA10.9900990099009901No Hit
TTATACTAGTTTTGACATAGGAGTATTATGTGTGAGCATAGTTATGTATA10.9900990099009901No Hit
GGCTCGCTCTGTTTGTGGGATTCGGCCAAGATGGGAGGTGTGGGCACGGT10.9900990099009901No Hit
CCTCTTTTCTGCCTGTTTGCCCACCTCGGAGTATATTATGAATATTTGCT10.9900990099009901No Hit
CTCGTATTGGAGTCAGGATATGGTCATGGGAGAAGAATTTATTGTGTTTA10.9900990099009901No Hit
CCAATGCATTGATAACACTCTTCTTCCATCCTCATAGCCCTAAATTAAAG10.9900990099009901No Hit
GTGCGGTGTGAACCTCCAGCCAGGCTGGGTGGAGGGGTCCTCCCGGAGTT10.9900990099009901No Hit
ACCCCGCATCGTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATC10.9900990099009901No Hit
ATGCTGTTATCCATCAGACTAACCATTGAGCATCACATCACCATACCACC10.9900990099009901No Hit
TGATTAGACTTGAGAAATAAATGATAATAAATATCCATGTTTCTAGTTTG10.9900990099009901No Hit
CCATAACACCCCTCTTTTTGATCATGTTTGGTGGCTCAATCGCACTAATG10.9900990099009901No Hit
CATTTAGAGAGGTCAAGTTCAGACTTCATGACCTTCTAGGTTCCCAGGGG10.9900990099009901No Hit
AGATACCACTGCCTTAGTGACTATCTGAGCTCTCTTTTCTCACCACGGTC10.9900990099009901No Hit
GCACTGCTGGGTTTTCTCTTTAGCTGTAAGTCCCTATGGGGGATGGCCCG10.9900990099009901No Hit
GTTATAGGAAGGCTTTAAACGGGTGTGAGGTGTAGGCTGGACCATTATCT10.9900990099009901No Hit
AATTCAAATAATATATGAAGAGTTAATATACAAAATATATAAAGAACTCC10.9900990099009901No Hit
GCTCTCTGCCCACAACGTCTTCTGTGAACAACCTCCTCCTTTTGTTCACT10.9900990099009901No Hit
ATTATAGGTTTGACAGTAGATTAGATGCCATCGACTGCAAAAAATTCTAG10.9900990099009901No Hit
GGGGCAGGGCCGAGGGCACCTCTTCGAGCTGGTTCTTCTCCATGTAGAGG10.9900990099009901No Hit
AAATAAGGTAGAGCATGCTCTATGAAGAAAAAGGTTATACCCAGTTAGGA10.9900990099009901No Hit
GGGAGTATAGCCCAAAAGTGGACTCGGCGGGTCGCTGGTAGCTCTGTGTT10.9900990099009901No Hit
CCCGATGGCTCATGTCTCCTCCCATCCCGCTTCGCGGACCATGCTGAGTT10.9900990099009901No Hit
CCGCACCAGCAGGAGACGCCCGGAGAATCCCACCCGCGGCAATGGCCGGA10.9900990099009901No Hit
TCTCTCTACTGCTCATTGGTCTGTTCATATTTCTGTTTCTTTAGGATTCA10.9900990099009901No Hit
CCTATATTCTCTTTAATGACGAGATGTTTTTGTTTTACTTTGGTATATGA10.9900990099009901No Hit
TATCGTGGGTGTTATAGAGTGGTTTCAATAGGCATTAACATTGGAGCATT10.9900990099009901No Hit
TTCTCTCTGAGAGAAAAGTGTCACCAAGTTTTCTGAAAACGATTGTGAAA10.9900990099009901No Hit
GCTCATCATACCAGCGTCCTCGGATCAAGTCCCACATGCAGATCCTTGCT10.9900990099009901No Hit
CTCAACCGACTGAGCCACCCAGGTGCCCCTCTCTGCAAGACTTATTTCAC10.9900990099009901No Hit
ATATTATGCTAATCCTAGCTATGCTATCTTAAAAAAATTTGGAAGTAACA10.9900990099009901No Hit
TGACAGATACACACAGAACCCTCCATCCAAAAGCAGCAAAAGGAATATAC10.9900990099009901No Hit
GTGTGGCGCGGTTAAAAGTCGGTTCAGCTATGGCTGTGGTTTGTGAAGTA10.9900990099009901No Hit
TACAAGTATAGCCTGTGCAGACTGACTAGGATGTGGGTCCTGGCATGGTC10.9900990099009901No Hit
GGCTCTTGGAGCTCAACCATACTAACCTTAGTTTCAAGGCTTACCTCCTC10.9900990099009901No Hit
TATGTACACAGTGTCCCTGTGATATGTGTGTATATGTGGGTCGTGAGTGG10.9900990099009901No Hit
ATTCCTGACAGCATGTGTGGTGTCGAAAATTCAAAGTCATCCCAAAGTGG10.9900990099009901No Hit
ACTTCCCTCTGCCCATTTTCAGGGGTAGAAAGATTAGTAACATCTGAGGA10.9900990099009901No Hit
GTTTGAAAAGAAATTTTAATAAGCAGTTAATAAAATGTATTTGAATTTTA10.9900990099009901No Hit
TACTTTTACGGAGTTACAACACTTATCTGTTGTAGTGGACTTCACTGCTG10.9900990099009901No Hit
GGTTTATAGAGACCACACACTGACCACCGCATTGATGTCGGTACATCTCA10.9900990099009901No Hit
GGCCATGCTGGCATCCCAAAATGAGGTGGGGAGCATTCCTTCCTCTTGTT10.9900990099009901No Hit
CATCATAATGAAAAACAAAAAAATGTCCGAATAGCTAAACCAAAAATTAA10.9900990099009901No Hit
CCCAGATGCCAGCCGGGCGCGCTTGGTTCTACGGGGATGCCTGCGATTCC10.9900990099009901No Hit
CCTCTTCAGGTACAATAGAATTTGGTTTCCACATATTAGGGTGAGCATTA10.9900990099009901No Hit
GAATAGAATACGTGGGAGGTGAATTTTTGTGAAACTGCATGTCTGAAAGT10.9900990099009901No Hit
AGCCGAAGGCCAACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAG10.9900990099009901No Hit
AGACTGTAGCATGGGTGGCTTAAAAAACATACCTTTGTTTCTCATGGTCC10.9900990099009901No Hit
GCATACTACAGGATAGGGAAAGTATACTATAAAACAAGGTAGCTGTACGG10.9900990099009901No Hit
GAGTGCAAATAATTCTAAAGAAAAAATATACACAGTGTTTAGTTAAAAAA10.9900990099009901No Hit
ATCCCATCCTCTGGCCAGCTCCCGGGAAGGATGGTGCAGGAGGAGCCTGC10.9900990099009901No Hit
CTACATGTTAGGACAAAGCCAGTTTCCACTTGAATAAAAAAGTATAGTAG10.9900990099009901No Hit
TGCCAGGAGCGGGGACTCAAACCAAAGGGAGACGCAGTCCCCGCTTGTCT10.9900990099009901No Hit
AGTGAGCTTCCCCCAGTTAGCACCCCGGCTCCCCGGTTGCATAGTTGGGC10.9900990099009901No Hit
TCTTTAAACAGAGCGGCCCTATTTGTCTGTAGGGTATGTGTGTGTTTCTT10.9900990099009901No Hit
GAGTAGGCTGACCTCCCTCGACACCCCAATACCTCTAAACTTTACTGTGT10.9900990099009901No Hit
GCATCTCTCTAGAGCCTCCTGTCCCCCATAGATGGCGCCAGAGCCCTCTG10.9900990099009901No Hit
CTTCTGGCGTCACTGCACCAGCCGCGAGGGCCGCCAGGAGGCCCTGCCCC10.9900990099009901No Hit
GAAAGAGGAATCATCATCAGAAATGAGACAGATGTTCCCTGAAACCATTG10.9900990099009901No Hit
TCTCTTCTCCCCACAGACCAAAGAGCTATATGGCGACTGATGCCAGTAAT10.9900990099009901No Hit
AGATTCTCTTTCTCCCTCTACTCTTCCCACCACCTTAATAAAAAAAAAAA10.9900990099009901No Hit
CCCTGGCTTAACCACAGGGAGACACAGAGATCTGTCATGAAGATTCCTGG10.9900990099009901No Hit
GCCAAACGCCCTGCCAGTTGTCTCATTATTGAGCACCAACTGCATGTGAG10.9900990099009901No Hit
GTAGAAGAATTTATTTTGGTTATTTAAAGAGAAAAAAAAAAAGCAAACCA10.9900990099009901No Hit
GGTATAAAGGGGAGCCTACAGAGAGGACTTCAGGGAAAGTTTTCTTCACC10.9900990099009901No Hit
AATATAAAACAGTATATAGTGTCACTGATAAATTTAAGACACAATTATAG10.9900990099009901No Hit
GGGCGAGGGTGGGGGCGGGGGGGGGTGTGGGGGGGGCTGCCTGGCGGGGG10.9900990099009901No Hit
GTCCACTGCCCCCTCATTTCGCCCTGGTCACATACGGCAGTCTCCACCTG10.9900990099009901No Hit
CCTTTAGTTACGGGAGTCTAGCGTAAGGCTCGGACCGTGGGACTTTATTT10.9900990099009901No Hit
GATTGTGGCTCCGGGTCACCACCTCACGAGTCGGCAACTTCAGAGAATGC10.9900990099009901No Hit
GTATATGAGAGGGGTCATAAAGATCTTTGGAGGCAACACAACTCTTCCCA10.9900990099009901No Hit
AATTATAGAAGTGCCAGGCACACAGCCCGTCTAAAATAAGGCGAAAAAAA10.9900990099009901No Hit
TAATTGAGCAGTGTCTTTCATATAAACATACAGAGATAATTATTGAATCT10.9900990099009901No Hit
TCCCAGTCCCTCTGTCGGTCCGGCTGCCCACCCCCCCCCGGTGGGCACCG10.9900990099009901No Hit
GAATGAAAGCAGGCCCTGGGGACAAAGCAAAAACAGACGAGTGTGCCTGT10.9900990099009901No Hit
TATCAAAATTAAGTAACGACTTGTTGTTGTATAAATTTACATTACAGAGC10.9900990099009901No Hit
CCGTCACCCAGACACTGGGTCCAGATGTAGCGGGCGACTGTGGATGCGGC10.9900990099009901No Hit
GTTCTACACGATTATTACGTCCTTGAAAAATGAATAAAATAAGTAAGGGA10.9900990099009901No Hit
CTCGGTCTCCCTCCCTCGGGGGACTCCACAGAACTGGCGGCACAGTCTGA10.9900990099009901No Hit
GTGGGGAAGCTCTCATAACTTGTCCTGGTCCCTTTATCTGCTACTTTGAC10.9900990099009901No Hit
ATACTGATCTGAGCTGGCGTCAGCCGCTGAACTGACTGCGCCACCCCGGC10.9900990099009901No Hit
GAACATTTGGTGGTTGCGCAATCATTTTCATAATTGGGCAGTATCTCCTA10.9900990099009901No Hit
GACGGATTAGTGTGTCAGTCGTGTTATGTGCTGAGGTCATGATGTCAGGG10.9900990099009901No Hit
CATTAAAAGTAGAGTTACTTAAGAGTATATGTTAGGAAGTTTATATAAGT10.9900990099009901No Hit
CTCCCCAGGTCAGAGGATCAGGAGAGAGCCTTGGGAGCAAATACCTCCGG10.9900990099009901No Hit
CGCACACGCTTTCTCAGTCTGTTACAATGGGTTCAGTTTATGGATTATAA10.9900990099009901No Hit
GGGTCATAAAATGTGAAAAAGTAAAAAAAATTACTTTAAAAACAATGTTG10.9900990099009901No Hit
GAACCGAGGAAGGAAATGGACCATATGGTTAAAACTACAGTCAAGTGGCC10.9900990099009901No Hit
CTCAGCAAACAGTCCTCAGATAAATCTATCCTCTTCCCAAGAAGAGAACT10.9900990099009901No Hit
GTTATATAGAACTTTGCACTCAATAGAGACTACAGGATGTTCTGGAACAT10.9900990099009901No Hit
ACCTAGAGCCCTCTGATTTGTGAAATTCAACAGAAAATATACTTTGTGAA10.9900990099009901No Hit
ATCTGGAAGGGGCCGGAGAGATCCCCCAATTCAGTTGTTTTCAGACTGTG10.9900990099009901No Hit
GAACAGGAGGGTGACTCTGGAGTGGTCAAGCTGTCTGCAGCAAGGACAGC10.9900990099009901No Hit
CCCCGTACCCCACCACGCCCCACAGGCCGCCAGGAGAGACACCGTTACCA10.9900990099009901No Hit
ATCATAGGCCACGCTCGCTGCTGAAGTGTATTCGCTGGGTCTGCTTACTT10.9900990099009901No Hit
AATTTATGCTCCTCCAGTGGATGAAGACTTCAGATACTAATGAGCCGTGG10.9900990099009901No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers