FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1797_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACCGAGGGCCTAAGCTGACAGGGCGCTTATAATACTCCACCACTCGT10.7462686567164178No Hit
GTGTGATGAAGAGTGATTTTCCCTTCTGCTTCTTTATCCTTTCATCTGCT10.7462686567164178No Hit
ATATAGATCACTTATCTGAAACTTGTGAAAGCACCTAGACTTACCCTCTA10.7462686567164178No Hit
TCTCCTCACCTCCTCCAGCGTCTGGTCCTTAGGAGGTTGGACTGCTGCCA10.7462686567164178No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGAAAAGCCAAATTCACTTAAATT10.7462686567164178No Hit
CTAAAAAATATTAACCTGCAGTCCATACGACTTCAAACCCCACACATACA10.7462686567164178No Hit
CCAAGGTGGTGAGCAATCTGTCAGGCTGTTGTCTTCAGGGACCTGCCATT10.7462686567164178No Hit
TGTTTAGTCACTCAATGTTTATTCACTCGAGAGAAAACAATTGCACATCA10.7462686567164178No Hit
CCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAGGAG10.7462686567164178No Hit
GCTGAACATGGGTCGTAGCCCTCCATCACCAGGACAATAAGATCATAACC10.7462686567164178No Hit
GGTCAGGACACAGTCCCTCGGCCCACCACTCGCCAGAGCTCCCAGGCCTG10.7462686567164178No Hit
AATCCAAACACTCTAACAAGAGCCAATGAAGGGCACTACAACTGATAATG10.7462686567164178No Hit
GAAGCAGGCTCCCCACTGAGAAGAGAGACCGATGTGGGGCTTGATCCAAG10.7462686567164178No Hit
GTTAGTTACTGGTCGTCTTCGCTGCTGGGCTGTCATGGGAAAGATCTGGA10.7462686567164178No Hit
CCACTGAGCACGAAAACACGAACAATCCAACAACTCACCTGAATAGAAGG10.7462686567164178No Hit
CAAATAATCCAATCAAGAAATGGGCAGAAGCCATTGACAGGCATTTCTGC10.7462686567164178No Hit
GGCTATAACCCACAGAGCCACCCAGAACCCCCCAAATGTTCCATACTTTA10.7462686567164178No Hit
CTCTTCTACTGACGACTGCTCCATCCACATTGCTCAGTCTGTCAGTCTCT10.7462686567164178No Hit
GAATGCCACCGGCCTGAGGTGGATCAACCTCGATAACAACCGGATTAGCA10.7462686567164178No Hit
GAGCCGAAGGCAGACGCTTAACCGACCGAGCCACCAGGCGCCCCCTTACC10.7462686567164178No Hit
AGTATGAACAATCAACCATAGGTCTCAAATGTCATAGGTGCGCCTCTAGT10.7462686567164178No Hit
GAGCTGGAGAGAGAGACAGACAGACTGAGCAAAGTGGAAGGAGCAGACGG10.7462686567164178No Hit
GTTTACTACTTGAAATTGATTTATAGTTCTATACCACTGTCGTTGGAAAA10.7462686567164178No Hit
GCCCATGGGCCACTGAGGACAAGATGAGCAGAAGTGGGAGGAGCCAGTAG10.7462686567164178No Hit
CTCTGAATTATGATTACAAAATAAATAAGGTAGTGCATGGGCTCTGAAGA10.7462686567164178No Hit
CTAACGACCTACCCAAAACTGGTTTGCAAATGTGCTTCACACCCTACTTT10.7462686567164178No Hit
GGAGACCCTAATACTCAAGTCCTGGGTTGTACTTATTTCTCCCTTCCTGA10.7462686567164178No Hit
CCTGCCAAGATTGAATCCTAAAGAAACAGAAATCTGAACAGACCAATGAG10.7462686567164178No Hit
GCCCACTGTGAATCCTGCAGAGGTATCCCGTGAAAGGAAATATGACGGGC10.7462686567164178No Hit
GGGAGAGACCCACTGGCAAAGAAGCTGCCCCGCCATCCCTACAGGGCCAA10.7462686567164178No Hit
TCCTGAGATCATGACCTGAGCTGAAGGGAGACCCTGAAATGACTGAGCCA10.7462686567164178No Hit
GAACAAACATTGCATCCCTGAAAAACATCTTAATAGATAATACCTCTTCA10.7462686567164178No Hit
GTAGTGAATGGGCTCTGAAGATACAGATTATACCCACTTAGGTGGGAGGA10.7462686567164178No Hit
TAATAAACCAGCACATTCATTAGACATATCAGAGAAAGAAAAAAATGAGA10.7462686567164178No Hit
GGAAAGAACCTTGGAGCCTTGTTAGAAACTGGAGAAGATCGGACCCACAT10.7462686567164178No Hit
CCTCCAGGGTGTCAGTGCAGCTAGGGGGTCAGCGTCTCCAGGAGAGGGTA10.7462686567164178No Hit
TGTAATCATCACTATTCCCATTTGATCTGCTCAATGATAATGAAAAAGGA10.7462686567164178No Hit
GGTTATAACTAATTAATAATTTCTACGAGAACCCTTAAAAGCACATTCAA10.7462686567164178No Hit
CAGCAGGGCTCCGTGCTACCCCAAGGGAGCTCTAAATGACAAATGTGAGC10.7462686567164178No Hit
ATTCAGTCTAGATTTTATTTGTCTTTATGCCTCTGCAGCTGACTCTTATA10.7462686567164178No Hit
GTTTATGCATCCCCCCCAACAGTTTATCTTCCCATGTATCCCCTTCGATT10.7462686567164178No Hit
GTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAGGCCAAGATGAGG10.7462686567164178No Hit
ACATAGCAACGTGTTCAACCTTGAGAATTTCTGGTATCTACTCATGTGCT10.7462686567164178No Hit
GTTTCATTTTCCACATGTGACTGAACACAGTTCCCAACACCAATGATTAA10.7462686567164178No Hit
TAAATGCAGCTAGTTCTTAGTAGAAAATTTCTCTTAACCCCCCAGCTGGG10.7462686567164178No Hit
GGTCAGGTATTGGTTAAGGCCTGTGAGGAAAGCAGAATGGTCTGACCGAA10.7462686567164178No Hit
TCACAGGGCGAAAGATCCCTATTGCTGCTCGGGAGGGCTGCAGGGAGGAA10.7462686567164178No Hit
CATCAACCTCAGACAACCCCTTACTTGGATGGACTTGACAGATACACACA10.7462686567164178No Hit
ATTTTATATATGCTTGTTAGAGAAAGCTTCAATAAAGTAGCCAACATCTG10.7462686567164178No Hit
GCAAATTGCTTCTCTGACTCCTATGAGCCACTCTTAACATAATAATCCAA10.7462686567164178No Hit
CTTAGGGAAAATCATTGTGTGACTAACTGAATGAATAAATAAAAATATCA10.7462686567164178No Hit
GATTCGTAGAATTAACAGGTGAAACCATGGGGTCCTGGACTTTTTCGTGT10.7462686567164178No Hit
CTAAAGATATGAAAATAATCAAAACCTTTCTGTGAATCTTAAATAAGTGT10.7462686567164178No Hit
TCACTGTACACAGCTCATCTCGGCAAAATGATTTACTCAGGCTGCACACA10.7462686567164178No Hit
ATAAACTGCTTATCAGAAAGTGATACAGCAGTGGTGTGCAAACAGGAACT10.7462686567164178No Hit
GGTCCCTACTTTAGTGCAGCTTTCTATAACAAGCCTATCTAAAATATCTA10.7462686567164178No Hit
ACTTCATAACGGAGCTACACGTGGAGTCCTTCCAGAATGACACCGGCCTG10.7462686567164178No Hit
GTACCCTTTTCTTTTGAATTCTAATTGTTGGGAAGGATGTGAACTCTTTA10.7462686567164178No Hit
GAAGTGTATATTAAATCTAGTTGCTAAAATATTAAAAAAATATTAAAATA10.7462686567164178No Hit
CTACAGATCTGACCTTAATCCAAGAAAAGCAGGTTCAGTTTCACCCTCAA10.7462686567164178No Hit
GGACCCCCCATTTCTGTAAATCACAGAATATGGGCTTGAACGGTGGATTT10.7462686567164178No Hit
GTCTAGAGATTCAGGTCTCAGCTTCTGACCTCTCCCTTTGTGTTAGTAGG10.7462686567164178No Hit
CTTTGTATACATCATAACACTTTTGTTACAAGCTGTAATCATTTTACAGA10.7462686567164178No Hit
TACCTTCAGCAAAACTTCATAACGGCGATCCCCGGGGAGTCCGGACACAC10.7462686567164178No Hit
GAGATCATTTTCTTCTCCTACTCATCCCAAAGGTGACCTTCCTATCACAA10.7462686567164178No Hit
TCTCGATAAGGGCCGAGCTGTCCTAATAAAAAGAGCTGGGTAGCGTATCT10.7462686567164178No Hit
TGGTTTGTCCTGATCATGTGGGCATAGAGGTTGTTTAGTGGGTGGGAGAA10.7462686567164178No Hit
GAAATATACCTCCCAGAATCAGAATAGGCTGATAAGCGATAGCAACGAGA10.7462686567164178No Hit
GAGCCAGAGAGAGCAGCAGGCTCCCCACTAAGCAAGGAGCCCAATGGGGG10.7462686567164178No Hit
GAGCCACCCAGGTGCCCCACCAAATATTAAACTTTTTAACTTAAAATGTA10.7462686567164178No Hit
GGGTAACCTTTGCCAGGTGATACCTGATGAGATTTGTCACCCCAGGGCTT10.7462686567164178No Hit
GCAGAGAACACTATGAGGGGATTTATCCCAGGACCCTGCGATCATGACCT10.7462686567164178No Hit
TCCCATCCACTATCCCTCTGTCTTCCCCTCAGAGGTAATGCCATTTTTTT10.7462686567164178No Hit
GTTAATTACATTATCAGTTGTAGTGCCCTTAATTGGATATTGTTACAGTG10.7462686567164178No Hit
ATTTGATTACAGTCCTAATTTGGGAAAATACTAGGCTTCTGGAATTTATT10.7462686567164178No Hit
ACATTCATGTTAAAATATAATAATAAATTCCCTGGAAGCTCAATATTATT10.7462686567164178No Hit
ATGTTAGTTTTATCAATGGGATTGCATAGCAGGCAGGACTTACATACCTG10.7462686567164178No Hit
TCATTAATGTCTGTGCATGGACCCACAGTTCCCAGAGTCAATTCAAAGAA10.7462686567164178No Hit
TCATTGGTCTAATTGTTGCTTTGGCTGTAACTTGGTTACTCAACCTGTGC10.7462686567164178No Hit
AACTTAGAAAGGGTGGCAAAAACCCACAAAAAGAAAGTCTCATAAACTCC10.7462686567164178No Hit
CCCCCACAAAAAATAATAAACCGCCCAATACTTTGTATAACTGTTACAAA10.7462686567164178No Hit
CCTGTTGTCTTCAGGGACCTGCCATTTAGGAAAACAGGTGAAAGTTGGAT10.7462686567164178No Hit
TCATTTTACCATTTGCTTAATTTTCTTTGTTTCTATTTTTTAATAAAGAT10.7462686567164178No Hit
TTACCACAGTGCTTTCACCAGCTTGCATTACCAACAAAACTGTACGAAGG10.7462686567164178No Hit
GATATAGACATTTATTCATCGATTCAGCCAGTATTTACTGGGTTCTTGTC10.7462686567164178No Hit
CTTCAAAGCACACAGTTTCACCCTCTCCGAACTGTGTCTTCAGAGAGTCA10.7462686567164178No Hit
CAGCAGAGACAGCCCAGCCAGGAGCACCCTCCGGCCTCCTTCCTCATCCG10.7462686567164178No Hit
GTATCATTTTCTTTTGGATTATCCAGCTTGTTGGGGTGTAATTGTTCATA10.7462686567164178No Hit
CAATATGTCAGCCTGTTGACTTCAGGGACCTGCCATTTAGGAAAACAGGT10.7462686567164178No Hit
ATGAAAAGGCTCATAAGAATGTACTATGAACAATTACACACCAACAAGAT10.7462686567164178No Hit
ATAAAAATGTTCTGGACTACATTCATGAATTCATTAAGGAGGCCTATTAG10.7462686567164178No Hit
GCCCTGTTTGGTATGTCTTTCTAACCTTTTCAACCTTTCTGTGTCCTTAG10.7462686567164178No Hit
GATTATGGTCAGATCTGAGGGACAAGTGGGGAAGGTTCAACTCCGACTCG10.7462686567164178No Hit
GGCATCTTACTCAGGATATTGTGGGCCAGGTTGAGCTGCAAGAGGTTCTA10.7462686567164178No Hit
GAGCCATAGGGTACTAAGCGGTCCATATGGAAGGGTCCTTAGAGATCCCC10.7462686567164178No Hit
GTTAATGAGACCACAAAAATAAGGAGCTTCCATAAAAAGTTAAATTTTTA10.7462686567164178No Hit
AAATATTAGGAAAATTTATCATCTGAAGCAACAGAAGAAAGTTAATGAGT10.7462686567164178No Hit
TCAATTACCTCTAAATGCATAACAAATTAATAACGGATCTCCCAGTGGAG10.7462686567164178No Hit
TCCCAGTAAAATTGGATCATTCACTGATCCAAAGTAAAAGGCTTTAAACC10.7462686567164178No Hit
CAAGACCGGATCATGTATACAGCATATACCGTTCTTTCAACGACCTCCGC10.7462686567164178No Hit
GTAGAAACAAGGTCTCAAGNACATAACNTAACANNACACCTAAAGGATAT10.7462686567164178No Hit
AACCGTAAGACACCAGTACTGTAAATCTGCCGAAGAAAGAGAAGTTTGGC10.7462686567164178No Hit
GGGCAGAAGGCTCAGCATTCTCCCTCTAAACCTGGCTCTAGTAAAGGGCT10.7462686567164178No Hit
CCCATACACATGCAACCCCTAACGGTACCATGCCATTAGTAAATCAAAAA10.7462686567164178No Hit
CAGTACAATAATCCAACAATTATAAATATATACTGCCCCCGCATGCAGCA10.7462686567164178No Hit
CCCTATGCGGGGATTGATCACAGGACCCTGGGCTCATGCCATGAGCCAAA10.7462686567164178No Hit
AATTAAATAAGTCCAAGTCCCAGTTACAATAACAACAAGAAACTCCTGTA10.7462686567164178No Hit
CTGTAATAATATTACTGATTTATATGTTAATTGGCATATCTTCCATCTTC10.7462686567164178No Hit
AGTCAGCTGCAAAAGACTGGGAGCAGGGGACAACAGTCGAAAAGGACTGC10.7462686567164178No Hit
GTTTACTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA10.7462686567164178No Hit
CTTGTAATATACTGTGCTGCTTCTGAGAAAACAGCAGGGCCTGTTAATTC10.7462686567164178No Hit
ATACTAATATTCAAATAAAACATAAGATGATATATAGCTATCTTTTAACC10.7462686567164178No Hit
GCACACATTTACAACTAGGCAGAAAATTTTTCCATATTTAACTTTCCTTC10.7462686567164178No Hit
CACTTATAGAAAAACATTCTGATAATAACTGAATGAATAAAACCAACATT10.7462686567164178No Hit
GTTTTTAACTTCTGTATTCATTTTCTTCCTTCTCAATCTAGCAAGAATAC10.7462686567164178No Hit
GTATTAGTCAAAATACCTCAGAAGTGGTAGGAAGGTTTTTCATAGAGGAA10.7462686567164178No Hit
GTTCATGGCAGCAAACCTAACAAATTATCTTATGATTATCAGTCAAACCG10.7462686567164178No Hit
TCCCAAGAGGGACCGCCTCTCCTGCACCTCTTCTTCCCTGACCCGAACGA10.7462686567164178No Hit
ATACTAAGTTGTAAGAATCATGAAAACAGTAAACAGATTATGTAGCATGA10.7462686567164178No Hit
GGGGCCCACAAAGGCAAACATTACCACAGCATTTCCAGGAACCACGGACG10.7462686567164178No Hit
CTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGGCGA10.7462686567164178No Hit
GCACTAAGCATAAGGAACATATGTCTTGTCTATTAGTGTTATCATATTCC10.7462686567164178No Hit
CTTCAGGAGAGTGCGCAGATCCTAGCTCCAGCAACTGTTCCAGAAGTTCC10.7462686567164178No Hit
GGCTCACCCTCACCAGAGCAGGCTAGGTCTTTGGCTGGGGGTCCCGACCC10.7462686567164178No Hit
CAAATTGTGCTCCAGAGTCGTGGTGGAGATATGAATATAAAACGCATTAA10.7462686567164178No Hit
GCACTATGGAGGCACAAGGCATGGAGACTCTGCAGCTCTGGGGCTCTCAG10.7462686567164178No Hit
GCCTCAGACAACCCCTTACTTGGATGGACTTGACAGATAAACACAGAACC10.7462686567164178No Hit
TTTCCTTTCTTTCCAGGACCTCCGCAAGCCCTGCTCTGGAAGTAAGGAGA10.7462686567164178No Hit
AGCAGGGGGAGTGGCACAGGGAGAAGCAGGATGCCCACAGAGTAGGGAGC10.7462686567164178No Hit
ATCCCTCACAGTCCCAGCTGCTCTGCCCACTAAAAGCAAATCTACCCTCT10.7462686567164178No Hit
CTCTACAAAGAAGATTTAAAAATACAGACTGCTAAGGTTGGCACAGGGCC10.7462686567164178No Hit
CATTGAAATTTTGGTAGAGGTTGCATGGAATCTGTAGATCATTTTGAGGA10.7462686567164178No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAAACTAAGAGAAG10.7462686567164178No Hit
ATCATGAACACCCAGAATCGTGTAGCATTTGACATATAGAAAAGCACTTA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers