FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1797_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6299918
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG323630.5137050990187492No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC294180.4669584588243847No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG256530.40719577619899183No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC254350.4037354136990355No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC185950.2951625719572858No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG165530.26274945165952956No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA155760.24724131329963342No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG154670.24551113204965525No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG138000.219050470180723No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA131520.2087646220157151No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA130670.2074153981051817No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT119620.189875487268247No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT110210.17493878491751796No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC104660.16612914644285848No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG96130.15258928767009347No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC92530.14687492757842247No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC87460.1388272037826524No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA85740.13609700951663178No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT85480.13568430573223333No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC84340.13387475836987084No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC83530.13258902734924485No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG83190.1320493377850315No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA81270.1290016790694736No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA80870.12836675017039906No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC78500.12460479644338229No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC78400.12444606421861365No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA77330.1227476294135892No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC76560.12152539128287067No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT76250.12103332138608787No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA75660.1200968012599529No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC71490.11347766748710061No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA70890.1125252741384888No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT70340.11165224690226126No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT69280.10996968531971368No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG69080.10965222087017641No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC68920.10939824931054658No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA68630.10893792585871752No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC67660.10739822327846171No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA66480.10552518302619177No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA65780.10441405745281128No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG65210.10350928377163005No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT64830.1029061013175092No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG64730.10274736909274057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA41000.0108.425912
GGTACTG41300.0107.0579765
CAGGTAC54550.080.7984853
GTACTGA59000.077.397656
AGGTACT63550.072.087184
AGTAGAA35950.071.470451
AACCGCG754.8639777E-967.65933145
GACTTAA2300.066.18848145
AACACTG43150.066.016275
CGCCGTT651.0950165E-555.763187145
GCGAAAG9250.055.663832
AGAAACA51600.054.229053
GACAGTA71300.053.778097
TTTGGAC73200.052.1907963
ATATCAC9800.051.040793
TTTTTGG76550.049.683051
ACTGATC107150.049.585488
ATCACTA10100.049.5184445
GTAGATG6050.046.7482872
CGAAAGC11150.046.1612853
Produced by FastQC (version 0.11.5)