FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1797_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938262
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC367490.3697729039544339No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG287230.28901431658774945No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG261140.26276224152673777No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA244550.24606918191530874No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA209230.2105297686859131No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT176560.1776568176608747No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG168980.17002972954426035No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT163480.1644955627050283No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC144880.14578001666689808No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG143110.14399902115681795No Hit
GTATAGCATGGTGGGTTCTTGGGACCATTGGATCTTTACAGTCTCCCAAT141360.14223814988978958No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA133470.13429913600587307No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG131650.13246782988816355No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG131450.132266587457646No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC126790.12757763882658757No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA120830.12158061439716522No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA119010.11974930827945571No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG118270.11900471128654085No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT117120.11784756731106505No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT113420.11412458234649077No Hit
GTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACCACCTT111170.11186060500316856No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC109290.1099689261563038No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC108680.10935513674322532No Hit
GTACATAATGAAGGGGGTTTACATAAATACAGCTTTGCTCAATGCATCTT106010.10666855029581632No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT105030.10568246238628042No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC104920.10557177904949577No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT101700.10233177591816357No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA101200.10182866984186974No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC100140.10076208496012683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA17750.096.834942
AGTAGAA26250.082.128551
GGTACTG20450.081.879595
GACTTAA3250.078.069824145
CCGTATA5700.063.59071145
AGGTACT29650.058.6760644
CGCCGTT1800.056.38376145
TATACTA32150.055.492752
CAGGTAC31550.053.537133
CGCAATT4450.052.13009145
GACAGTA45050.051.3283427
TTTGGAC44300.051.05633
AACACTG25800.050.2910045
AGAAACA44350.049.034544
GCGAAAG7900.048.655382
GTACTGA41800.047.688476
TAACTAT24700.046.1391641
TCTGACC10900.041.899857145
TATAGCG36550.041.867512
GCGGTAT37400.040.507156
Produced by FastQC (version 0.11.5)