FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1797_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1797_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4509259
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC85860.19040822449985687No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG73980.16406243243069427No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA63660.14117618881505808No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA50250.11143737807032153No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT45780.10152444115540936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG13850.036.6514132
GGTACTG22250.035.509645
AGCGAAA14800.034.328111
AAGGTCG9850.031.6443719
TATAGCG5100.031.2820342
AGGTACT27000.030.0686324
GTATAGG51600.028.9755121
GACAGTA24600.028.2872527
GTACTGA33300.026.9918146
GCGGTAT5700.026.7053976
GCAGGTA27850.026.559352
CGAAAGC20300.025.3545233
CCGTATA4700.024.679123145
GTCGGTA36100.024.098032145
GTTCTAT33600.023.9771331
TAACTAT12750.023.908521
TCTATAC46150.023.8761883
CAGGTAC31700.023.7830313
GGATAGC25400.023.4007595
ACTGATC48150.022.2804958
Produced by FastQC (version 0.11.5)