FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4932640
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA18800.039.3712841
GCGAAAG20200.036.974432
TATACGG4600.033.089655
CGAAAGC23000.031.8319953
TACGGGG4900.031.0643837
ATATACG6250.025.5149864
TAGGGCG2404.375681E-521.140615
GTCCTAT35850.020.2417531
TAGTGCA43900.019.8142789
GTATTAT54200.019.6813681
GTATATA31200.019.5371841
GCACCGT16500.019.3287546
GTGATCG5407.4578566E-1118.7929869
ACCGTGC8950.018.6278158
GGTAGTG48000.018.2718967
TATATAC29000.017.9971943
TATACTG22200.017.6307775
TAGGACT37050.017.6078784
CTAGGAC22750.017.524743
GTATAAG19550.017.44571
Produced by FastQC (version 0.11.5)