FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5925950
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG29500.029.011442
AGCGAAA30450.027.173981
CGAAAGC33550.025.0665843
TATACTG35900.024.2310685
GTCTACG3702.564775E-1023.5404781
GCACCGT42350.022.0811656
GTCCTAT45250.020.8526131
AGCACCG43750.020.711795
CACCGTG57550.019.3981867
ACCGTGT48700.019.3510158
CCGTGTT51000.018.1940429
CTAGTAC15850.017.8387763
TAGTACT22300.016.9043644
TACACCG16650.016.5445545
TTATACT34500.016.3898854
TAGCACC69950.015.9600184
ATAGCAC69800.015.891533
GATCGTC2750.002639237615.8162777
GTGATCG8750.015.7410949
AATCGCG3757.52559E-515.4648057
Produced by FastQC (version 0.11.5)