FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4549837
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG23750.031.4561212
AGCGAAA25500.029.0355991
TATACTG33000.027.897395
CGAAAGC31600.023.6317313
GTAATAT28900.021.6008781
GTATTAT40250.020.7397141
GTATATA22250.020.2270471
GTCCTAT36400.020.1414531
ATATCGA6600.019.770026
TATATCG6051.8189894E-1219.170725
TACACCG13300.019.0761265
TAGGACT43800.018.702194
TAACCGT4104.6332934E-717.6803135
GTAGGAC33850.017.5630763
TAGTACC16950.017.5348594
TAAGGTA33150.016.8381844
CTAACCG7351.8189894E-1216.766784
ATACTGT60200.016.4969126
CACGCCG3554.7109963E-516.3356135
TAATATA38350.016.2653982
Produced by FastQC (version 0.11.5)