FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4461536
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC75600.16944836935082447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG18550.028.9340042
TCTATAC55850.028.6860473
CCGACGC9600.027.94162145
GTTCTAT60750.026.6446841
CTATACC66900.023.9465754
GTCCTAT39000.023.078581
ATACTGC24400.022.875136
CGAAAGC23700.022.3293533
TATACTG40400.021.8897675
GCGTTAC3706.5756467E-921.5795781
TATACCA77050.021.449885
TACCACT70550.021.0629657
ACGTACG2104.321434E-420.711394
TAATACT32900.020.0503884
TACGTAC2556.9688715E-519.9002933
AGCGAAA27150.019.516641
CGTACGG2608.0960934E-519.5158445
GAGTATA59250.019.454937145
TAGTACT18700.018.9946694
ATACCCG5759.094947E-1218.9097635
Produced by FastQC (version 0.11.5)