FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3338797
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG55460.1661077328151427No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT50500.15125208271122803No Hit
TCGTGAGACAGTTCGGTCCCTATCTGCCGTGGGCGCTGGAGAACTGAGGG37180.1113574739644249No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA37160.11129757214948977No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG15200.054.8430569
CATAGTG20800.042.1717834
GTCATAG23700.036.418312
CGAAAGC23150.034.4482883
TAGTGAT27800.031.5510986
GTGATCC29400.030.8193578
AGTGATC30000.029.4789837
GTGATCG2603.7325663E-927.8800839
ATAGTGA31850.027.5391085
TCATAGT32850.026.9246713
GATGCGC24500.026.0361948
GGTCATA35650.025.8581431
GATCGAT8000.025.3712567
AGCGAAA32250.024.3078861
TATACTG18900.023.779565
TATACGA1902.1884036E-422.9043922
TCTATAC21250.022.1758563
GCGAAAG36400.022.117842
TACGAGT2653.7871814E-621.8848724
TATGCTG24600.020.921715
Produced by FastQC (version 0.11.5)