FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4298873
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC52540.1222180790174541No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG10200.038.3776329
AGCGAAA24100.037.0431941
GCGAAAG23950.036.639232
CGAAAGC25950.034.363533
TCTATAC35600.032.3800243
GTTCTAT40350.028.2407651
GTCCTAT27800.026.1081181
TAGACCG5900.025.8025074
TATACTG23200.025.6212525
CGTACGG2751.9745494E-723.72385
CTATACC47850.021.8160044
TATACCA47950.021.7694955
CGGTACG1700.00309664521.3209049
TACCACT46650.020.822497
CTATACT14700.020.7122214
GGGTACT12000.020.53897
GTATACG3551.01686965E-720.422163
CCGTATA5700.020.349556145
ATACCAC51750.019.891046
GAGCGAA8500.019.6394461
Produced by FastQC (version 0.11.5)