FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1796_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1796_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCCACTGATCATTAATCAGGCACTCCTCAATTGATCCATATAGCCCT17.142857142857142No Hit
GCCTTTACCATGCAATCTTCTTGTGAGAACACCATTGCCACAATAATTGC17.142857142857142No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG17.142857142857142No Hit
CTATATTAATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACT17.142857142857142No Hit
GTATTCCCCTTAAACGCAGCCATAATGGTCGGTCTTTCGAAGGGAAGATT17.142857142857142No Hit
CCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAGGAGTGCCT17.142857142857142No Hit
NNATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACT17.142857142857142No Hit
GGTCTCATATACAACCGGATGGGAACAGTGACCACAGAAGTGGCTCTTGG17.142857142857142No Hit
CTTAGGGACAACCTGGAACCTGCAACCTTTGATCTTGAAGGGCTATATGG17.142857142857142No Hit
CACTAAGAGGAGTGAGAGTTAGCAAAATGGGGGTGGATGAATATTATAGC17.142857142857142No Hit
CCTCTTCACCTTGTTATATAGATTGTTCAAGTTCGCATCATGCTCATCGA17.142857142857142No Hit
ACCCCTTTGTTCTTTTGAAAGTGAACCCTCCAAAGCTGAAGGATGAGCTG17.142857142857142No Hit
ATCATATTGAGTCTAGTTTCAACCTCACTGAATTCATGATCAATTATTTC17.142857142857142No Hit
GGGGAAATGCTATTGCGGACTGCAATAGGCCAGGTGTCAAGGCCCATGTT17.142857142857142No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)