FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103174
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3250.051.3502921
GCGAAAG3400.049.0492252
CGAAAGC4350.038.3286363
CCTATAC2553.0286174E-928.4278893
GTGATCG2104.9985283E-727.6119089
TACACTG7700.020.7079935
TATACTG6600.019.766725
CATATAC6750.019.3309633
TATACTA3001.1085958E-519.327465
TACACCG3001.1085958E-519.327465
CACCGTT1900.005898610719.073157
TATACAG6750.018.253715
CCGAACT2000.00791803518.124422145
TTAGAGT6102.1827873E-1117.8248754
TAGACCT4908.623829E-917.7521214
TATAGAC3703.3960368E-617.636972
GTACAGT5602.4629117E-916.8252456
GTCCTAT8550.016.1244871
ATAATAC10350.016.1091373
TAGACAG7251.8189894E-1115.9951385
Produced by FastQC (version 0.11.5)