FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1795_d12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAACAGGGAAAGGAGGAGGGATGAAATCGGGAGTGAGTGACTACGT11.2195121951219512No Hit
GTAAATAAACAGAATAAATTCTTCTCCAATGACCATATCCTGACTGCCAA11.2195121951219512No Hit
TACCAAACCTCAAATGGAAACCAAATCCTGTACCTGAGCACTGGATCCAA11.2195121951219512No Hit
AATCTAAGCTCCTTCGGAAGTCCAAAGGGCTCTGGGTTCCAGCAGAGTTT11.2195121951219512No Hit
GGTCACCCCACCCGGTGCTCCCCGCGGCGGGTGGTTCCTGCTGGGGTCGG11.2195121951219512No Hit
AACTGACTGCGCACACATGGAATCCCGATCAGGTCTCTTGCTGGATTGTC11.2195121951219512No Hit
AGCTTAAACCAAAGATAGTAAAAGAGGAACAAAGACATCAATAGGGACAA11.2195121951219512No Hit
ATTTCCAGTTTTCTTCTTGTAATTGATTTGGAGTTGTATAGCAGTGTTGT11.2195121951219512No Hit
CAAAAAGTAGATATGATAATACCAGATAGGACAACCACAATAAAATAGAC11.2195121951219512No Hit
TAGTAAGGTGTAATCACAACATCACCTTAAGTCATAAGGTGGGCCACAAT11.2195121951219512No Hit
ATTCTGTACAGTGTGAGCCCACAGATACTCAGAAAGTGGGCGATGGGACT11.2195121951219512No Hit
GTAGCAAGTGGTTCCTGGACATTATTGTTTGCCAAAATGGGAAACAAAGT11.2195121951219512No Hit
AGCTAGCATCCGCTCCTCTTACCAAATTATGGAATCTGAAGCGCGGGGAA11.2195121951219512No Hit
GTCTAATATGGGGCTCGGACTCCTGACCCGGAGAACAAGCGTTGCACGCT11.2195121951219512No Hit
GGATAGGCGACATAAAGTCATAAATCCATCCCTGAGAGTGGGAAATCGAC11.2195121951219512No Hit
TTAGTTAGAATAGTGGAAGATGACAAACAATTGCATGCCTCACATAGATC11.2195121951219512No Hit
CTATTACCCCCTTTTTATGGCTTTGCATCTTCTCCTTACTGAGACAAAAG11.2195121951219512No Hit
GGAATCAAGTGATCATTCTTAATTGACACTTTTATTCTTTCTTTAATTAC11.2195121951219512No Hit
CACCAGAAAGGGTTGGCAACACCGCGAAAAAAGCACCTCCGCGGCGGGGG11.2195121951219512No Hit
GAAGATGAGATCTCCCTGGGGACTTGATCCCCCTGAAGGGTCGTTCGAGA11.2195121951219512No Hit
GACTAAGAAACAAGCAAGAAGACAAAATACCAATATTAGATGTAAAAGAA11.2195121951219512No Hit
CTATTCTGCCCGGTTCAAGTCCCAGATCAAGAGGCGATAAGCAAAATCAT11.2195121951219512No Hit
CCCTCAGGCTGCTGCTTCTACCACAATTTCTTCTGGCTTTTCTCTCTTTC11.2195121951219512No Hit
CATATGGGGTGTATCCAGTCGCCGCCTCCTGCAGTTGGAGAAAGGCAAAC11.2195121951219512No Hit
ATTCAATCAATCTTACTATGAAAATGGTATAGTTCAACTCTAGTTACTCT11.2195121951219512No Hit
GGAAGAGCCTTGGCACATAGAAACGTAAAGAAAATCTTGGCCACAGGGCC11.2195121951219512No Hit
AAACTGAGCAGTTCTGTTTCCACCATCCAGGGGTGGTCCCTTCCCAATCC11.2195121951219512No Hit
AAAGATAGGAGAAATACACTCCCCTGAGGGAACATTTAGTCATTTTGTGG11.2195121951219512No Hit
CTCCTGAGAGGTGCACCGGGCCCGGTCCCAGCGTATACCGCGGGTCACAG11.2195121951219512No Hit
GCTGGGTGCTGGGTGGCCAGCTGTGAGGAAACTCCGTGCCCCAGGCCCAT11.2195121951219512No Hit
ATCTTGGGGCGCCTGGGTGGCTCCGCGGGTTCCGCACCTGCCTACAGCTC11.2195121951219512No Hit
ACTAAGATGATTATCAGTTCAACTTTGATTTGGATATTAGCAGTATGCTT11.2195121951219512No Hit
GCCATGGTCACACTGGTACAGGGCCCACCTGAATGACCTCATATTAACTT11.2195121951219512No Hit
GGGTGAGGCCTCAGAGGCCCGCCCCCGCCTGCATCCCATGGGCGCACACG11.2195121951219512No Hit
TGGCAAAGGGCAGTTTGAAAACATAGGAAGGTGGCCAAGCCTGGGTTTGT11.2195121951219512No Hit
CTCCTAAGTAGTCTTCAACTCTTGGAGATCATGATGTATATGTCAAGGTT11.2195121951219512No Hit
AGGCACGATTTCTTCGCTTCATTGTGCTCCCATAGAAGACTCAGCCGTTA11.2195121951219512No Hit
GTGCCCAGAAGGAAGGCAGCTGGCCGACTCATAGCAAGAGAAAGTCTTTT11.2195121951219512No Hit
TCGCTCTTCCATAAATATGACTAAATCTATTGGGCCATTTTTCTGTGGAG11.2195121951219512No Hit
CTGTCTGCCTGTCTGTCTGTCTCTGTATTTTGTCCTGGGAAGATGATATT11.2195121951219512No Hit
GTCCAGGCCACAAGACGATGCCAGGGCCTCAGGTCTTTCTGACTGCACGC11.2195121951219512No Hit
GTTCTAGACTGCCCTGGAACAGCACTGTGACCTTAAGCAAATTAGCCAGT11.2195121951219512No Hit
CGCATAATACTCCATAGAGTATATCGACCACAACTACTTCATACATTCAT11.2195121951219512No Hit
GGGTGGCAGTGCTTTGGTCGCGGCGCTATCTAATGGTTGTTCTGCCTCTG11.2195121951219512No Hit
ACCTCAAAGTATTTCTGTGTTCCTATTTTGGTTTGCCCATGAAGCCAATT11.2195121951219512No Hit
TTCCTTTGTGATCTTCTGTCTGGATGGGGGATCATTGAGGGAAGTGTGAG11.2195121951219512No Hit
GCTCCAGGCCAGGCTCTGCCCTGCCCCCACGCCGCCGCATTGTCTGTAGG11.2195121951219512No Hit
GTACATTGCATGCTTGTTCCAATGCAACTGCACAGTGTGGATTTTTGACA11.2195121951219512No Hit
AAACTCCTCGTTTTAGAGTCTTATATTGCTCTGTCTTATGACAAAGTAGT11.2195121951219512No Hit
CTATAGGACTAATAAGAATCAAGAGGGAAAAAACCTAGTAAACCAAAGAA11.2195121951219512No Hit
TTCTCATGCTTTCTGAGTCAAGAGTTAGGGAAAAGCACGTGGCTGAGAGA11.2195121951219512No Hit
TAATTATGTTGAGCTGCTGTGGGCAGGCCTAGGAGAGGCATTCAGCGGCG11.2195121951219512No Hit
GTTTAGCTATGTCCACTATCTCATCATAGGGAGAAAAGAGAAGAGGAATT11.2195121951219512No Hit
ATGTATCGCCGCGTGTGCACAAATGTGCACCTGTGCCCACTGGGGTCAGA11.2195121951219512No Hit
TCATTTGTACGGGGTGAAGGGAGGGTTCCAGGACAAAGCTGGGCTGGTAC11.2195121951219512No Hit
GTATTGTGAGACAGCATATGGTCATTAGAGAAGAAATTATTGTGTTTATA11.2195121951219512No Hit
GAGGGGTGGCCCCTAGTGCTCTGGAGCCTGGCCCTGGGGCCAACCGGGGA11.2195121951219512No Hit
ACCCGGGGAGTACGCCTGGGGGGGGACCTTACATTCTTACTTGCCCTCCG11.2195121951219512No Hit
ATGCGACAGTGTGCGGCACCAACCACTTTGCTAAGTCAGGCAAGAAGCCA11.2195121951219512No Hit
GGTCCCTGCCTGCCAATGTCTCCGAAATAGTTTTGAACCACTGACTTCTC11.2195121951219512No Hit
TTGTTGAGTAGCATGTTTTTTAGTCTCTGTGTTTATGAATTTCCAGTTTT11.2195121951219512No Hit
CTTTAATATTTAGAAACGATATGGAGCACCATCACACTAGAAAGAGAACT11.2195121951219512No Hit
AGCCAAAGGTGGCTACAAGCTGTTCAGAGCCCCCTTACCGGGGCTTGAAC11.2195121951219512No Hit
AACCAAACATTTGATATGACAACCAATCCCGACGCACAAGGAGACTGGAC11.2195121951219512No Hit
ATCCAAGGGATTGATCAACACCGGCATCATGACCTGAGTTAAAGGCTCAT11.2195121951219512No Hit
TAGTAGAAGTGCTGGTCCCTCTTTATCAGTGCACTCATCTGGGAGAGCCT11.2195121951219512No Hit
GTCTTGCTTCGCTCCTGACTTTTCGTACCTCACAGCAATTAGTCCTACTT11.2195121951219512No Hit
AACTAATATAACAATCAAACAAAAGTAAAACTACAGCACAGATAAACTAC11.2195121951219512No Hit
CTCCCCGCAGCAGCCCTGCGAGCACTCGAACGGGGGCTCCTGGCGCGCTT11.2195121951219512No Hit
GACCCCCAAGATCATGACGGGGCTGAAACCAAGAGACTGTCACTCGGCGG11.2195121951219512No Hit
CCCATATACTCGTCTAAGAGACTCACGCTACTCAAAATAAGCCGCAAAAA11.2195121951219512No Hit
AAAAAAAAACAAGAAGAACAATAACAGATCAACAAAGAGCTGGTTCTTTG11.2195121951219512No Hit
CTCGTACGGAGTGCTGAGGGCGGCACCGGGTGTTCCCCCCCCGCAAACAC11.2195121951219512No Hit
TGTGAGCAGTGGTTAAAGCATGCCCATATCCTTTACCAGTGACTAACTCT11.2195121951219512No Hit
ACTAACTCACTCTGCAACCCAGCAACCCAATCAAAAAGATTAAATACAAA11.2195121951219512No Hit
CAAGAGGAGACACCACCATTTCTGTTGAAAGTATATAACGCAGGGAATAT11.2195121951219512No Hit
GCTGAGCGCCCTGTCCGCGGCCCGGGCGGCCTCCGGTCCATGCCCGCCGT11.2195121951219512No Hit
GTAGTACGGGGTCCTGATTAGGCGAGGTTTAGACATGCTCAATGCATGAT11.2195121951219512No Hit
GGGATAAGCGTTCGGTGAGAGACCCTGTGGGGCCCCGACCCCCTGCGATG11.2195121951219512No Hit
GTGGTGGAGAGGGCTGCGAATAAATACTCCTGTCACTTACATAAAACCTG11.2195121951219512No Hit
GGGTGTAGGGATGGAACACTGGTCCCTTGTATCGTTGTGTATATTTGTGT11.2195121951219512No Hit
ATATATAATCTGTCAGGATCTGTAAAAATCAAGTAAAAGCCCATTATACA11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAT50.0145.05
TAGTTAG50.0145.02
AGAATAG50.0145.07
AATAGTG50.0145.09
GTTAGAA50.0145.04
GAATAGT50.0145.08
TGAATCC50.0145.0145
TTAGTTA50.0145.01
AGTTAGA50.0145.03
TAGAATA50.0145.06
Produced by FastQC (version 0.11.5)