FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3955487
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG6850.031.748089
GCCGTAA1751.2570029E-424.856094145
GCGAAAG16050.024.4001352
GCCGACC5350.023.036413145
AGCGAAA17650.021.7960761
ACGCCGA3157.3038973E-720.7110586
TATACTG24600.019.1535195
ACCGACG2659.367971E-519.1484418
CGAAAGC20600.019.0035723
GTCCTAT36150.018.4725421
GACCGAC3302.5183075E-517.5730177
GTATAAT17050.017.4544681
TACGCCG2500.00139583817.397295
TACACCG8400.017.2592145
GTATAAA38300.017.2460541
ATTATAC15150.017.226563
TATAGAC14100.016.973382
TATAATA33150.016.8453752
GTATACG6053.7289283E-1016.7965371
TAGTACT19050.016.7440664
Produced by FastQC (version 0.11.5)