FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1795_d10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGACAAGATCCAAGGGCAGTAACACTGTTAAGTATACTTCTCACCCCCC11.098901098901099No Hit
GGCTGGGGAGACAGGACACACCAGGAGGAGAATGCCGTATCTGACCAGGG11.098901098901099No Hit
AATCCCAATCTATGATTCATCTCATGCACATGTCAGTAGAAGCACAAGTC11.098901098901099No Hit
CAAGAAACATCTCAAATATGCAACCTAACTTGATACCTCAAGGAACCAGA11.098901098901099No Hit
AGGATATACTGCCCAATTATGAAAATGATTGCACAACCCCCAAATCTTCA11.098901098901099No Hit
GAATAAAGTAGTTTAATCTCTGACTTTATGAATATACAATATACTTCCTT11.098901098901099No Hit
TCTGAACAGACTAATGACCAGTAGCGAGCTTAAATAAGAAATCAAAAACT11.098901098901099No Hit
GTGTGGAAGACACAGTCACCCTTACAAGTACCTTCTCAGCGAGATTAAGA11.098901098901099No Hit
GGGGGTCACTGCCTGAGCTGGCCAGGTGCTCTGCAAGAGCCCAGGTTTTT11.098901098901099No Hit
TCCCAGTGCCGCCCCCTGGGTCTCCCTCCCTCGGGGGCCTCCACAGAAGT11.098901098901099No Hit
GGTAAGAATAGGTGATTGCACAAGTTTCAGAGAAGTGATCTATATTTATA11.098901098901099No Hit
AAGTTTCACTAATTCCAACGATCTACCAAAATACACAACCTAAGCAACAC11.098901098901099No Hit
CACTGACAGAGCCACCATGTCCACCTAAACCTTGACCTTACTTAGGTGTC11.098901098901099No Hit
GAGAGAGTGAGAGAGAGAGAAAGAGAAAGAGTCTCAGCTTTATTAGTTGT11.098901098901099No Hit
CTCAAGACATAATCACAGGAACCTCGGATTAAGTCATGACCCAAAGGCAG11.098901098901099No Hit
GCACACACGTGCACGCACACACACTTCTGAGAGCACTGATGGGAAAACAT11.098901098901099No Hit
AATTTGGATAACGTTCTAGGTGCACTTGAGAGTAGTATATTACTTTTGCT11.098901098901099No Hit
GAGCAACCCAGGTGCCCCTAGCCTCTTGCTTTTACTTTAATATATCCAGT11.098901098901099No Hit
GGGCTCAAATGGACTCCCAGCTTGAAATTATGGCTCCCCTCAGAAAATTC11.098901098901099No Hit
GAACCATCCATCCAAAAGCAGCAAAAGGAATATCCTCCTCTCAACTGCCC11.098901098901099No Hit
GCTTAATCATCCCTCCATTTTCCCGCGGCTGACCTGCTTCCCGGCCCCAG11.098901098901099No Hit
ACCTTATTTAGTTTGTAAACATAATTCAGAGTCAGTGTGGCTGTGTTTTA11.098901098901099No Hit
CAGTAAAACAGGTGCTAAAATGGGGCACCCGATAAGTAGATTAGTCAAGT11.098901098901099No Hit
CTAAGATAATCTTACTCATTTCTTCTCTAAGGTATGTAAATATATGCTCT11.098901098901099No Hit
CTTTTGTACCCTCTTATACTAAGAAATTAAGACTGGCCATCACCTTCCAA11.098901098901099No Hit
GGATGAGATTGTATGGTAGCATCGACCACAAGAGACCCATTCATGCAGCT11.098901098901099No Hit
ATGAGCGACAGAGGATAGAAGGACAAACTTCTCCAAGGTTCTCCCCACAC11.098901098901099No Hit
CACATAGAAACTAACACATTCCTCAGAACCATCTCGATGTCTTATAGAAG11.098901098901099No Hit
TTTCTCCTCCCTGGACTAGAGCCTAGTCTCTCTGGAGTGCTCTCTCTATG11.098901098901099No Hit
GCATTCCATCCTCTTCATAGACTGGCATAGATCGAGAAGGATTGGTAATC11.098901098901099No Hit
CTCCCCAACCCTGTGCACTCAGCAGCCAAAACGCAAAATCCGCCTGAAAT11.098901098901099No Hit
GAATTGAGTGAAATGGAGATAACTTAGAATAGTACCTATGAGAGTCAGCC11.098901098901099No Hit
GCCCTGGGCTGCCACGTGGCCGCAGGAGCTGAGCAGGTGAGCCGCGCCCC11.098901098901099No Hit
GCATGGGGAGGAGCAGAGTCAGAAGCAGGTCTCCCGCTGATCAGGGAGCC11.098901098901099No Hit
GTGCTTGTCATAATTACTCCAGAGCTCCACAGCAGACTCTAGAAACGAGG11.098901098901099No Hit
TACAAAAGACTAAGTTTAAGTCAATGTACATGAAACCGAAAATGGTCAGC11.098901098901099No Hit
GGAAGAAGAAGAGGAAGAAGAGGAGGAAGAAGAAGAAGAAGGAGAAGGAG11.098901098901099No Hit
GTGCAATTGTGGGAAGGTTTGATTTCCTGGGAGCTTCTGGAACATTCACG11.098901098901099No Hit
GTTTGGGACAGGTGGCCTGGTGTCTGTTCACCTTGGGTGAGCTCATTCAG11.098901098901099No Hit
GAAATTGTGCTTGAGATTGGTGTTGGAAAAATGAATTTAAAAAGCATTAA11.098901098901099No Hit
GTAAAGGTGACACACCTTCAGGGAGGAGACACAGGAAGTTACTAGCCTGC11.098901098901099No Hit
TAGATGCTCCTATGATGTTGATCCTTAGTTTTGTCAATGCGGCACATTAC11.098901098901099No Hit
GCTGTGTGGAGGGCAGCTCTGGGAAGGGCACCCCCCAGAGCTGGCCAGGG11.098901098901099No Hit
GCCATATTCCTCTTCTCCCAGAAATTATATATATTTAATAGACCAATATT11.098901098901099No Hit
CAATATAAGAAGGAAGTATATTTAAGGAAATGTATCCTTTTCTTTAGGAT11.098901098901099No Hit
GTTCTGCACAACTGAGAATCAACTCATCAGAGCAATACACCTGTCTATTT11.098901098901099No Hit
GTTTTGGAGATAATACGAATTAATAGAAGGCATAAAGAACAAACCTGTTT11.098901098901099No Hit
GTATTGGGAGTCAGGATATGGTCATTGGAGAAGAATATATTGTGTTTATT11.098901098901099No Hit
ATTTCAGTCCTCATTTGGGAAAATTCTAGGCTTCTGGAATTTATTGACTT11.098901098901099No Hit
GACACAGGGAGAATATCGTCATTTACAAGCTAAATAGAGCAGCTTCAGAA11.098901098901099No Hit
ATTTTAAACTTAACTTTAGGAATAAATAACATTTTAATGTGAACACTTAC11.098901098901099No Hit
GGACCCTGAATCTCTGTACTTTCAGTGCCCCGTACGTGATCCAGGTGAAC11.098901098901099No Hit
ATTAAGACACTTTCCATGTCAGTGTTTTCATGGAACATCTCTTTCATTTA11.098901098901099No Hit
GGGGCACGCTCCGGGTGTTTTCACTCCACTCAAGGGAGCTCTGGCCACTG11.098901098901099No Hit
ATCTTCAAATATGGGCACAGATTTTTGAGGAAAGGGATTTGGTCAAGACA11.098901098901099No Hit
TTTCTTTACATTTCCATTTTTCTGACACTTAGGGAACACAAGATATTTTA11.098901098901099No Hit
AGGGTGGATTTCAAATCATTTTTAACACCACGGCACTTCTTTCAAATACC11.098901098901099No Hit
AGAACTTAAATAAATTAAATACAAACTAAAAAGACAACAGAAAGATATAT11.098901098901099No Hit
GGATTTTCCATATAAAGAATCATGTAATATTAAAAGAGATAGATTTTGAC11.098901098901099No Hit
GTCTGACAGATTGTCTCTCTCTAACTCTCTGTGTCAAATAAATAAAAGAC11.098901098901099No Hit
GCAACTAATAAAATACCATCCACTAGAGGGTCCGCCCACCCACCCAAAGA11.098901098901099No Hit
GCATAGATTTAATAAAAGTCAGACCTTCAAATCAATAAAAAAAAAAAAAA11.098901098901099No Hit
ATAAATCAGCGACAAGGTATCCTCACAAACCCCAGAGATGACCAGCCACA11.098901098901099No Hit
CTGGATTGGGGAACAGAAAACAAGATCAGGTCTCTCGCTTACGGGAGCCA11.098901098901099No Hit
CTCTTTCTGTCTCTACAATAAATAAATATGTCCTTAAAAATCTTATCCTG11.098901098901099No Hit
CAGGAGGCCACACTCATCCCGAGAGCTCCTGCTGGCTCCTAAGGATGCTC11.098901098901099No Hit
GCTGTATGTTCACTGAAGGAACGGTCCCTCAAAGCAGACACTCAGAGAAA11.098901098901099No Hit
GTATTATGTACGCCATTTCCCAGTAGTGTGAACCCACTTTCAATAGATGG11.098901098901099No Hit
GAGTGGGACCAGGAGTGAAAGGCAGAGCAAGGCCAGGGGCTGGCGTTGGT11.098901098901099No Hit
ACGTTCCCCCTTACTGGCAAATCCTGTCAGTATTAAAGCAGTTGCAAAGA11.098901098901099No Hit
ATGTACCCTTTTCTTATGGATTATACAGCTTGTTGGGGTGTAATTGTTCA11.098901098901099No Hit
AGATTACACTGCTCTATAAGAGATTCTATTACTCAGTTGGATATACTTAA11.098901098901099No Hit
GTGTAGCTAGCAGCTCATTTGCTAGTTCTGTTGGGTGAATTTACTATTAG11.098901098901099No Hit
AGATTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAGGAACCC11.098901098901099No Hit
GATGATGAGTCCTGTTTATGTGATTATGGGTGAGAGGCAGTCAGCTTTGG11.098901098901099No Hit
GTATAGACATTTTAACAATGTTTATTCTTCCGATCTAAGAGCATGGAATG11.098901098901099No Hit
CTAAAACTAAGTTAGGGATTGCTTAAATAGAAAGATAATCACATAAATAA11.098901098901099No Hit
CAACAGCAAAGATTTTGTGTCTTACAAAAATAATTTGCCATGCTTCACGA11.098901098901099No Hit
CTCATGCACCCATCAGAGTTCAGATGATAATAAGGATGAATGTATGGTTA11.098901098901099No Hit
GCCTGGCTCCGTCTGCACTAGGAGACGGTGTGGGGTGTGCTGGTGATGTC11.098901098901099No Hit
TCTCGGGACCGTGCCAGATGGTCCGCTTAGGACGCTATGGGGCCTGTGTC11.098901098901099No Hit
GTTATATAGAGGGTGACAATCTGCTTATGCTTACATGGGATTGGCTGTCC11.098901098901099No Hit
GGGATGGGGAGGGGTGAGACCACTTGTTTGAGAGCCGCTTCCCTCTCTTT11.098901098901099No Hit
TTGAGAGAAGTGTCAGCAATTAAAGTTAAAGATGGTATTGTTGATCAATG11.098901098901099No Hit
AATCTGTGTTCCAACCAGGAAGCCGTAGACAATCAAGCCTCCCTCTGAAA11.098901098901099No Hit
GTATTTATATCCCTGACCTAGGAAGCCAAGGTACCTGGGACTTGCCATCT11.098901098901099No Hit
GTCTACCCCTTGCATCGCTGCTCTTACTAGGTTTTTTAAAGACACGGGGT11.098901098901099No Hit
ATGGCACCTATCAAGCAATTCAACTTTTCTTGTAATGTCATGTCATGACT11.098901098901099No Hit
ACCCCACACAGAGTGGTGAGCCCAAGCAGGGCTTGATCTCTCAACACTGA11.098901098901099No Hit
GAAATACTGACCGTAGCATCTCTAAGGCAACATATACGTGATAACACATG11.098901098901099No Hit
GACATGAGCTGAAGCCAGGTGCTAAAACAACTAAGCAACCCAGGTGTCCC11.098901098901099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGATT50.0145.06
GACAGAT50.0145.05
TGACAGA50.0145.04
GTCTGAC50.0145.01
CTGACAG50.0145.03
GATTGTC50.0145.09
TCTGACA50.0145.02
CAGATTG50.0145.07
CTAGACT50.0145.0145
AGATTGT50.0145.08
Produced by FastQC (version 0.11.5)