FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3868548
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA16950.032.975211
TATACGG2202.2717359E-829.6537515
GCGAAAG22400.025.5811272
TATCGTC2051.2931834E-524.7519637
GCCGACC4750.024.419182145
CGAAAGC24700.023.7737883
GTGATCG9050.022.4277739
AGATCGT6650.021.8005896
GTATCGC2704.457257E-621.4776158
TATACTG21850.021.2318785
TTATTCG2404.3696185E-521.144215145
AAGATCG7100.019.3979175
ATAAGCG2256.875499E-419.3321043
GTCTACG3051.2631639E-519.039551
CCGTATA2000.00792357318.123611145
ATAGCGT2000.00792829818.1217388
CGTAAGA8150.017.7888984
AGCACCG22050.017.7519055
TCTATAC48700.017.5656173
GCACCGT24450.017.491746
Produced by FastQC (version 0.11.5)