FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1795_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTACTCTTGCCCTCATTTGTGGACGTGCACAAAGTGGTGAGAAATTT23.7735849056603774No Hit
AACTACATCAAAGAATTTTGGTTTCAGGGTAAGACACTGATCTTATCCTC11.8867924528301887No Hit
GGCCAAGATGAGGAGAAGTGGGAGGAGCCAGTAGAGGGATGACCTCATGG11.8867924528301887No Hit
NAACAAAAAGGTCCCCCGGCCCTGACAAAAGGACCCTTTGCCGAGAGGGA11.8867924528301887No Hit
CACTGATAAGACCACAAAAATTTGTGTACTTGGAAAACAAACATCTTAAA11.8867924528301887No Hit
GAATTCCAAAACTATACTCAGCGCCTGCTACTAGGCATGACAAGGATAAA11.8867924528301887No Hit
GTGCTAATAGTCCTGCTATCACCATGCTTCTGCTTTTCATTGTGATCATA11.8867924528301887No Hit
AGCAGTGCCACGTCGGTTTTTGGGAATGAGGACCTGCATGCTAATACAGT11.8867924528301887No Hit
AGACCCAGTGGTCGCAATAATGAGACCATTCTATTGATTAGCACACTGAA11.8867924528301887No Hit
CGAAACATCCACCAGCAAGATCGCGATGAACACACCACAACAATCCTAAA11.8867924528301887No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCAGGTAC11.8867924528301887No Hit
TTATAACCCATGAACTCTTTCTAAAAGACTAATGTTACGATAAGAGTTAT11.8867924528301887No Hit
TCTCCACACATATTAATAAAAAACATTAGCCACCTGAGCAAGGAATCTGA11.8867924528301887No Hit
GTGTAACGCGTTACTGAATTGTTAATGAACTGTAGTCTCCTCAGTGGCAA11.8867924528301887No Hit
AAATATCTCATTAAGAAAAAATGAAACCCCCTCCTCAAATCAGAAGTGAT11.8867924528301887No Hit
GTAATGGTCACAGAAGGCACAGTCACTTACAGTAGCCACAGAAGGGGAGC11.8867924528301887No Hit
AAATGAACCAGGGCAAAACCAGAGAGGCAGAAACACAATAAGAGATTCTT11.8867924528301887No Hit
TCTCAAGACCAGGAGGCAGGGCACCTAGAAACCTACCTCTTCTCAGTTCT11.8867924528301887No Hit
GGGAAAGATCCATAGAATCCAGTTCCATTTGTGCCCCCAGCTCATTCAGT11.8867924528301887No Hit
TGCTTTAAGAGCAATCTCAAATGGTACTTCTAGGCTTCCAAAATGAATAC11.8867924528301887No Hit
GGTTGGTATATGGGGATAAGGTAGATAAAGCCACAGGGAAAAGACAACAT11.8867924528301887No Hit
ATTCTTAACACATTAGTTAAAAGCTGTAATCATTTTACTGATTTCTATGT11.8867924528301887No Hit
ATTGCAGGGATTTTACAAAAGTTCCGTCCTGATCTCTTCTCAGTAAGGCT11.8867924528301887No Hit
CCCCATGCTGACCAGGGACCTCATGCAGGACTCAATTCCAGGCCCCTAAA11.8867924528301887No Hit
TCCTCATGCTGGGCCCTCCCCTATTCCTCCTTCAAGGCCCACAGCCCATG11.8867924528301887No Hit
CGACTTCTCTGAACCCCAGATGCAAGCAAGACCTTCCTGGCCACCTACTC11.8867924528301887No Hit
ATGAAAGTAAGCCCTCAAAAAATGTTGTAAAAAATCTTATTCTGGGGGTA11.8867924528301887No Hit
GTTCTGGACTCTGCGTCTGGCGGAGTGCCCAGGCGCCAGGGTGGGTCTGG11.8867924528301887No Hit
CTTTTCAGATGTCTAATAATGACCAGTAGAGAGATTAAATAAGAAATCAA11.8867924528301887No Hit
TGAAGCGGGGATGTGAGGGTCCAGGGACTCCCCGCGCCCCCCCACTACGA11.8867924528301887No Hit
GGAGTAGTGACTACGAGAGCTAGAAGGTTGGGCAACTGTGAGCAGAGCAT11.8867924528301887No Hit
GTTTGTGACCTTACACTAGTTAAACTATATTTGTGGTAGTGTTCCATCAT11.8867924528301887No Hit
CTACGGACCCTTCCAGATGGACCGCGTAGGGCCCTATGGCTCATGTCTCC11.8867924528301887No Hit
GAAGAAGGCTATCCACAGAGCAGGGACTGCAACGTGGGGATCGATCAAAG11.8867924528301887No Hit
CAGACCAATCGAGGACCAAAGCGTCGTCTCACCGACCTCAGTCGTTGTCC11.8867924528301887No Hit
TTTCACCTTAGTGCATACGGGGGAAAAAATCCAGAGATGTTATCGCCCAT11.8867924528301887No Hit
GCCATCTCGGGTGCGTGTGATGCGGATTTACCGATCACTGGGGCGGGACG11.8867924528301887No Hit
GCAGAGGGTGTGGGGACCAGCCCGGCGGCTGAAGCAGCGGGCGGGGTGGC11.8867924528301887No Hit
ATACTGTACTCTGAAAGGCGTCTTTATATGAAACAGAACAAAGAATAAAA11.8867924528301887No Hit
GCTCAAGGCAGAAGCCCCAGCTGGGGGCCGTGCCCACCTTGTACAAAGAT11.8867924528301887No Hit
CTTATGATGGGTTTATGCCCCGATGAACCCAGTGTAAATTGAAAATGTTG11.8867924528301887No Hit
ATTTTAAGCCACTCAATTTCTGCTTAACTTGTCACAGCAGCAATAAGGAG11.8867924528301887No Hit
CTTTAGCTAGTGGTGTATATTTCTCACATTTAGCATCTATGAACTTATAA11.8867924528301887No Hit
GCAGAGTGCCATCCTAGGCCACGCACGCTGCTGCAGGGTCGAGGCTGTGT11.8867924528301887No Hit
CGATCCTGTTGCTGTCCCGGTAGAGCTGGTGAATGGCCGAGGGCACCTTG11.8867924528301887No Hit
CCTATAACACACTACACGTACCCACACTCCCACTTCCTTCTACCCCCACG11.8867924528301887No Hit
TCCCTGATGAGATTTGTCACCCCAGGGCTTCCTCGTGGACTAGTTCGCGC11.8867924528301887No Hit
CAACAGTTCCATCCCTAAGTATAATACATGCAACAGAAAGGCATACGTAT11.8867924528301887No Hit
GACTATGCCTTTCATAGCTGCTCTTAGTTGGTATTTTAATGTGACAGGGT11.8867924528301887No Hit
CCTCCTTGCTTGGTCACCAATCTCGGGTCCTTTTGTTTCAGTCCGCCTGA11.8867924528301887No Hit
CGACCGGAGATACAGCACCTCTAGCTGAGCTGGCTCTCAATCTGAGTATC11.8867924528301887No Hit
GGGGTGATAGTATGACGCTGAGATTAGGACCTGCGCTTAAATTAAGAGTC11.8867924528301887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGGT50.0145.01
CCAACAC50.0145.0145
TATATGG50.0145.07
TGGTATA50.0145.04
GTATATG50.0145.06
ATATGGG50.0145.08
GTTGGTA50.0145.02
TTGGTAT50.0145.03
GGTATAT50.0145.05
TATGGGG50.0145.09
Produced by FastQC (version 0.11.5)