FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4907750
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC67920.13839335744485765No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC55340.11276042993225001No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG5050.031.5804169
GCCGACC6600.028.558968145
CCGACGC8700.028.331705145
CGAAAGC22400.026.5391963
GCACCGT35150.024.3354176
TATACTG28600.024.0790675
AGCACCG37550.022.3939235
GCGAAAG27250.022.358962
CACCGTG44650.021.1059067
TCTATAC61900.021.081613
ACCGTGT41550.020.7614678
GTTCTAT63450.020.5945241
GTAATAT21050.020.3474861
ATAGCAC56650.019.9639553
CTATACC64800.019.3536134
AGCGAAA30950.019.2337531
CCGTGTT44200.019.1889029
TATACCA65400.018.288935
TAGCACC53300.018.0890334
GTATATA25350.018.0415331
Produced by FastQC (version 0.11.5)