FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d04_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d04_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4463052
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC70700.15841177741151122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG19150.037.8685532
TCTATAC45700.035.215473
CGAAAGC22450.032.6138233
GTTCTAT50100.032.598781
AGCGAAA22000.032.334041
ACCGTAC3300.030.7529248
CTATACC59750.026.083634
ATACCAC60350.026.064246
CGATACG1958.774419E-626.0214144
TTCTATA65250.025.2285822
TATACCA66150.025.0939045
TACCACT61850.024.7292087
TATTACG3757.75799E-921.2720322
GCCGACC6600.020.870161145
GTCCTAT36450.020.7105871
TAGCGCC2857.1177E-620.3473455
CCGTACA6650.019.6213119
ACCACTG78650.019.2628218
ATTACGC4553.446985E-919.1190593
TATACTG26700.019.004195
Produced by FastQC (version 0.11.5)