FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1795_d02_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d02_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444219
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC198240.18980835235262683No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG2654.5783963E-927.3528395
GTGATCG13650.026.5537059
TCTATAC154750.024.7349973
GCGAAAG50000.023.6443792
CGAAAGC51650.023.4398443
GTTCTAT147000.022.6136471
GTCCTAT100100.022.2600841
AGCGAAA55400.021.7480751
GCCGACC15700.021.70287145
TATACTG58400.019.9830285
CTATACC172050.019.717884
GTATAGG26950.019.660141
TATACCA186850.019.3577885
GTTGACA67900.018.3857421
TTCTATA189600.018.3617152
TACCACT174100.017.945177
GGTCCTA107900.017.0647549
ACGTACG4256.75338E-717.0561164
CTAATAC66750.016.942673
TCCTATG133300.016.8127462
Produced by FastQC (version 0.11.5)