FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1795_d02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1795_d02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62369
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG790.12666549086886114No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC770.12345876958104188No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGAC450.00957356848.363322
CATGTTG450.00960401848.3245055
GTCCAGG1000.006013138729.0179921
GTATTAA1050.00763082527.6361851
CTGGAGT1050.007660830427.6140044
AGGACCC1750.003624739620.7105035
TCCTGGG1950.00674627118.6012781
CAGGACC2000.0078434918.1216894
CCAGGAC2050.00904294217.6796973
CAATGGA650.00798446513.382171125-129
AATTTCC1008.554258E-411.5978830-34
Produced by FastQC (version 0.11.5)