FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794eye_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794eye_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4362665
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC5850.038.41635145
CCGTATA4200.034.521603145
GCGAAAG14300.031.9473082
AGCGAAA16700.027.8107071
CGAAAGC18650.025.2661933
GTGATCG6050.021.5676339
TACACCG5400.021.4783845
TATACTG31600.020.8751565
GTATTAT49400.019.9783921
GTCCTAT38250.019.9207841
TAGTACT18800.019.2795774
CTAGTAC12450.019.215423
GTATAAG22300.017.8980771
GTCTACG2550.001582110417.0749571
CCGACGC3453.685336E-516.81052145
GTATAAT26450.016.461681
GATATAT28550.015.7591981
ACGACGC2850.003347054815.262182145
TCCTATG42300.014.9144982
CTGCCGC13300.014.717105145
Produced by FastQC (version 0.11.5)