FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d12_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d12_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5252596
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG31000.032.7330745
GCGAAAG21100.025.0902422
GTGATCG6950.025.0315789
GTAATAT25500.023.9104861
AGCGAAA20950.023.9064121
GTATTAT60400.023.674381
GTTGACA31450.023.5412121
CGAAAGC24350.022.027943
GTAGCAT65900.021.8086641
TACAGCC40400.021.3500297
AGTCGAA3809.03492E-920.9929522
CTACTGA53950.020.5553346
TATAATA58500.020.3307062
ATACTGT50750.020.2804186
AGGTCGG19850.020.0838748
TAGCATG62900.019.8308932
CCGACTA8050.019.8088684
TAGTCGG6300.019.5593158
GTGCGAA3751.8164428E-719.3286514
TTACGTG7250.019.0055312
Produced by FastQC (version 0.11.5)