FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d10_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d10_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922258
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG48170.1228119108941839No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA39860.10162513531746255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT4200.041.4837151
TTACGTG4150.040.197772
TCGCCAT12450.036.68359145
TATAGCG3200.036.2653772
GTTGACA27400.029.9394361
GTAGCAT54750.028.2430251
TATTGCG1306.47029E-427.8964442
ACATGGT29000.027.494135
TAGCATG51700.027.0762792
CGCTATA3555.456968E-1226.5605562
CCGACTA7400.026.4473934
GCGAAAG13500.025.7887132
TATACTG21650.025.7797155
GTATTAT51750.025.3912431
ACGACTC3451.09139364E-1025.2120085
GGATAGC9850.024.2841555
CTACTGA42050.023.615956
AGCATGT60000.023.4378593
GCATGTT61350.023.1575344
TATACGC3854.292815E-1022.5934459
Produced by FastQC (version 0.11.5)