FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d08_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d08_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7493418
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC379370.5062709700700001No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA240950.32154885794439864No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG233290.3113265535166996No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC191500.25555761069247707No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA167550.22359622804973647No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT141030.18820516885618818No Hit
GGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCT138110.18430841573231335No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG130610.1742996320237307No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT124790.16653281586587054No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT122020.16283623841616734No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC121290.16186205013519864No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG117970.15743149521353272No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC113470.15142622498838315No Hit
CCGCTATATGATGCGATAAAATGCATGAAAACATTCTTCGGCTGGAGAGA112340.14991823490962333No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC109770.1464885583588157No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC108970.14542095476323355No Hit
TGGTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCC107050.1428587061338364No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG105580.14089698452695418No Hit
TAACTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATC101850.1359192827625524No Hit
TGTCCATACCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGT98570.1315421080206656No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT96180.12835264227886392No Hit
GTATGGACACGTTCTTTCAGGAGGGAGCCATGGAAGAATCCTGAAGACTG95130.12695141255966236No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG92890.12396212249203234No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA91890.12262761799755464No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA91720.12240075223349345No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT91640.12229399187393523No Hit
GTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCC89570.11953156757036643No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC89520.11946484234564253No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT84540.11281900996314366No Hit
CCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCACAT81620.10892225683926882No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA80230.10706729559194482No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC80040.10681373973799406No Hit
TGTATGCCCCACACGAAAAGAATGCTCTTTCCCCTGTTATTTGTGTATTG79390.10594631181658357No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG78820.10518564425473129No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT78790.10514560911989695No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG77470.10338406318718642No Hit
TCCATGCACTGATCATCACATTTATGGTATAGCTCGAAACAGCCTTTCCC76300.10182269292864751No Hit
AAGCTATTCTCTGTAATTCTTAACCTATGTCGCATTTCTTCAATTAACCA75730.10106202536679523No Hit
CTCCTGTAGAATGAACCTGAACATGCTCTGCTTGTTCCAGTGTAAGTCAC75720.10104868032185046No Hit
GTCTAACAGCCAATGAGTCAGGAAGGTTAATAGATTTCCTCAAGGACGTA75320.10051487852405938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA19850.0101.951772
GGTACTG19600.0101.704625
AGGTACT27100.074.89954
AGTAGAA23900.072.896021
AACACTG42200.071.2852555
GTACTGA33950.064.05396
GACTTAA2200.062.611332145
AACCGCG702.3372013E-762.14057145
GCGAAAG8600.057.3537062
CAGGTAC35050.056.8809283
GGATAGC41150.051.4371725
TTTCGTT3200.047.576378145
AGAAACA40250.045.3896373
TCTCGGG803.737802E-545.310837145
TAACTAT35300.044.0077861
TCCAACA71700.043.5011142
TATAGCG43500.043.187852
GCCGGCG855.3459888E-542.645493145
ACACTGT79100.042.337926
GCTTATA4300.042.1718442
Produced by FastQC (version 0.11.5)