FastQCFastQC Report
Thu 10 Sep 2020
HHH35BGXF_n02_1794_d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACATTACAGCCAGCAATCGTACCGCAAGAAAGATGCTGGGGCTGAGC11.25No Hit
ACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTA11.25No Hit
CCCGCATGCAGGGCTCAATTCCAGGACAATAAATTCATGACTTGAGCCAA11.25No Hit
CCTAGTACGAGAGGACCGGAGTGGACGCATCACTGGTGTTCGGGTTGTCA11.25No Hit
GGGCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGC11.25No Hit
TTGGAAAGATACAAGAAGAAAGGAATAAAAGTTCCCATAGAACACCACAT11.25No Hit
ATCACATCCTGGGGCTGAAGTAGGTCCCAAGGGTATGGCTGTTCGCCATT11.25No Hit
GCCGTTGGTGGGCGTTAACGGTGTAACCCAGCGGCCCGATATCATCGCTG11.25No Hit
GTCCTCTCTAAAGTTTTTATTTTATTAATTAATATAAAAATGAAAAAATT11.25No Hit
GTATCGAGAGTCAGAAATCCCCACGCGGAGCAGCTTCCAGGAGCCACACA11.25No Hit
CTGTCTACCAGAAGGGAAGTCTCAGGAATCAAGATGGCCTGCAAGATGGC11.25No Hit
CAGTATCCACAGATGACAAAAATGTCTTTGGCTGAAATGCTTTTCTAACT11.25No Hit
ATCAAAATGCACTTGTTTGAATTGATACTGGGCATTAAGTGGCCCCTGGG11.25No Hit
CTTAAGAGCCTTTGCATATCAATGGTTTCAGGGAACATCTCTCTCATTTC11.25No Hit
GGTGCATAATTTAATGACCAGACGCACGAGAAGTTGGCATCAAAAAGACG11.25No Hit
CTTAGAATGAAATGGATGATGGCGATGAAATACCCGATCACAGCTGACAA11.25No Hit
ATTCAAGGACCATTTGCTCTTGCGCACAGCCAACGGCAGCGGCTTAACCC11.25No Hit
ATATACCCCCAAACTGGAGATGCTGGATGATATGCTAAGGCTATTTTTAG11.25No Hit
CCGTGATACCATAGATATGGAAAGGCTCTTAAGAATGTACTATGAACAAT11.25No Hit
CCCGTGAGGTGGGGGGAGTGCACTCTGGGAAAGCTCCATAGAATCCAGGT11.25No Hit
CTCATAGCCAAGACATATGTACCTTATGCTTACTGAAGAATTATTTACAG11.25No Hit
TCCAAGGACAGCCCTGGCTAGAGAGAGCTCAGCTTGTCCACAGAACGGCG11.25No Hit
GGTATAAATTTATTAAAAATTATTTAACTTTTAAATGTATTAAAAGTCCT11.25No Hit
GCTTTATTTTCCTGTTGTCTTTCATCCTGTATGATCACAGGCCCTAAAAT11.25No Hit
CAGTAAAATTGTAGCTAATTTAATGGCCCATCGAGAGCGCATGCTCAAAC11.25No Hit
GTCTCTGTGTTTATGAATTTACAGTTTACTTCTTGTAATTGATTTCTAGT11.25No Hit
ATCTCTAATGGTCATTAAATATCACACAAGTAAGTTTTAAACAGAATATG11.25No Hit
GAGAGAGGTAAAGCGACTTGCCCAAGGTCACACAGCAAGTATATGTAGCG11.25No Hit
GCACCGGAACTGACAACCAACGGGAAGTCGCAAAATGCGCAGGCGGTTGG11.25No Hit
GTCTGGCTCTAGGCTGGTCCCACTGTTGGGAAGCACAACAGGAAAAGGAA11.25No Hit
ATGGATGCGCTGTTTATCGCTACTGATGTGTGCGGCTGCGGCGTGGGTCG11.25No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT11.25No Hit
TGTCATGCCAATGTCACTGCCCGGGAGCTAATTGCGGATGAGCTAAGTGC11.25No Hit
GTGATTGTTTGCTAAAGGGATGCCAAAACGATGTTACTTGAGCATCAATA11.25No Hit
ATCTTGTATACTTTCACACGTATGAAATTATGCCTTATTACTAAGGACAA11.25No Hit
GGTGAGAGTCTTAACCATTGCTGGAAGAGGACAATTGAATTGGCTCTATG11.25No Hit
CACCATGACTGATCAGTCAGCACCCACGCTGGGAAAGGCTCCCTCCGATC11.25No Hit
CTATGAAACCAGAAGGATAAAAAGATTAGAAAGTTAGAAGAAACATTGCA11.25No Hit
CTCTCTGCCTGCCTCTCTGCCTACTTGTGATCTCTGTCTGTCAAGTAAAT11.25No Hit
ACCGTGAGGCTTAACCTTACCACGCCGCAGGTGTGTTGGCGGATGAGAGC11.25No Hit
GCTATTTCCAGTCTGAGATACTATGTGACACTGCTTCAGAGAACCTCTTT11.25No Hit
CAGCAAGGCACTGCCCCTGCCACCGCCCACTACAGAACACAGCAAACGAA11.25No Hit
AGCCACTCCAGTCCCAGCGTTTGCCAGCTTTTCGGTGCGCGTTTTACGTG11.25No Hit
GCGATGATGTGCCCCAGGTGGGGCCCAAGAAGCAGGGTATGAATCTGGCG11.25No Hit
ATGTTTTGAGTGTTTCACCTCTCTAGACATTCTGCTGCCTCCAGGCTGTC11.25No Hit
GGTTTTCTCATTTTCTGTCACCTCCACACTCCGTTGTATGCCCCACACGG11.25No Hit
TATTCCAATGAAGAATTCTGGTTGTTGACTGTTCTTCTAAATGACTGTCT11.25No Hit
TAATATCACTAATTATGAATAATGGTCTATGCTCCAAGATAGGACTTAGC11.25No Hit
CACCACATCTTCTGTACCTTCTCCTCTGTGTCAGGTTCAGAGGATGGCTC11.25No Hit
GTCATGCCAATGGCACTGCCCGGTAGCTAAATGCGGAAGAGATAAGTGCT11.25No Hit
AAATAAGGTAGTGCATGGGCTCTGAAGAAACAGTTTATACCCAGTTAGGT11.25No Hit
TTCTAACGTAGTCTTTTCCTTGAAACTTTCTCACCTTCAACATGCAGGCT11.25No Hit
TGGGAACGGTATGTAACATACGTCATCACAGAGCGTGGCCGCTTTGGCAT11.25No Hit
GCCTAGCGCTCTTCCCCCAGTACCTGGTATGGTGCCAGGGCCAAAGAGTT11.25No Hit
AATTAAGTGTGTCGCTTCGGATGCGTTGTACGAGGGTCTCGTCGTTAGGG11.25No Hit
CGATTTGGGGACCCCGTATCCGGTGCTGAATCTGGCGTAGGTGGGTGAAT11.25No Hit
GCTAATCTGCAAAATGACCTTGGGGTGCTTTTCACAGCAATGCAATGGAC11.25No Hit
ACAAAATTCTGTGTATGTTACGCGTGGTAGGCAGCCTCTAAGATGGCCCG11.25No Hit
CTTACGACAGCATCCGTGCACCGCATGATCTCAGCGTGTAGGTTTTGGTC11.25No Hit
CCCACGATTTTTTATCTGCAGAGTATCGCGCCGAGCTGGAAGCGCTGGTT11.25No Hit
GTTCAGCCAGGAGTGAATGACGCCGTCACCCGGACGCAGCGCCACACCGC11.25No Hit
CGACAAATAAGCACCACAATGCATTTGATGTAAGGTCAATATCACACCAG11.25No Hit
ATTCAAATACATCCCAGTGTGAGGAATGCCATCATACCTCCTCTTGACAT11.25No Hit
TCTTACCTGTGCCAAGGAATACTTCACCACGTTAGGAATCCTAGTAATAA11.25No Hit
TTTAATGCATGTGCGACTAATAAGATAATTGGTTCAAGCATTTTTTTTTT11.25No Hit
TATTCCCCCACATACGCCAGATTCAGCAACGGATACGGTTTCCCCAAATC11.25No Hit
ACAGAAAGATATATATTAAAAAAAAAAAAAACAAGAAGAACAATAACAGA11.25No Hit
GTCTTTCCTCTTGGAATACTGCATCAACATTCAGGAAGCTTAACAATCTA11.25No Hit
ACGTGGGGCTCGATCCCAGGACCCTGGGATTATGACCTGAGCTGAAGGCA11.25No Hit
TTCTGCCCGTCTCGCAGTTTTCGTGGTGCCTCCTCCGCTCCCGCCGACGT11.25No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT11.25No Hit
GGAAAACCGTAGCACTTAATACCGCAGGTGAATTGGTAGTGCCGAACGGT11.25No Hit
GGGTTACACCGTTAACGCCCACCGTCGGCCCATCTCCAGTTCTTTGCTGG11.25No Hit
GCATAATACTCCATAGTGTATATGGACCACATCTACTTCATCCATTCATC11.25No Hit
CAATTACAAGAAGAAAACTGGAAATTCATAAACACAGAGACTAAACAACA11.25No Hit
GTGTGCACACTCGTGTGCATGTGCCTGTATGCATGGGTGTGCTTGTGGGT11.25No Hit
TATTTATACTTCTTGTTTTTATGGATTAAATAAAACTTAGCACCGTCTCT11.25No Hit
TGTATAACCTAGCTTGAGAAGCCATCCACCTTGAAATGCCAACAGGAGCA11.25No Hit
GCTAATGATGGGCATGGACCCAAAGCAAATAGCACGCGTGGTCCGTCAGC11.25No Hit
ATCCTGATGTGTGCGGATGCGGCGTGGGTCGGATGGTGGTGGAGCATGCG11.25No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCAT50.0145.09
ATCTCTA50.0145.01
ATGGTCA50.0145.08
TAATGGT50.0145.06
CTCTAAT50.0145.03
TCTAATG50.0145.04
CTAATGG50.0145.05
AATGGTC50.0145.07
TCTCTAA50.0145.02
ATTTTAA50.0145.0145