FastQCFastQC Report
Mon 14 Sep 2020
HHH35BGXF_n02_1794_d06_replicate1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHHH35BGXF_n02_1794_d06_replicate1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7109815
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC698620.9826134716585453No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG572700.8055061910893603No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG543950.7650691333037498No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA347990.48945014743702897No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT308050.43327428350808No Hit
GTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAA306750.4314458252429916No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG300390.42250044480763566No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC213410.3001625218096392No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC203880.28675851622018295No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA203510.28623810886781165No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA202900.2853801399895778No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA183480.2580657865218715No Hit
CACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAA175170.2463777186888829No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG175140.24633552349815008No Hit
TTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACA169390.238248111941028No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA162690.22882451934403358No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA157340.2212997103300156No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG155880.21924621104768546No Hit
GTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGTTCAACAGCC145230.2042669183375376No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG144780.20363399047654546No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA143650.20204463829227626No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC135440.19049722109506365No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT130510.18356314475130506No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT129670.18238167941078634No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA125250.17616492130948555No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG122710.17259239516077424No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT122610.1724517445249982No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA122300.17201572755409247No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT120290.16918864977499415No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG118930.16727580112844007No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC114920.16163571063382098No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTG114480.16101684783640643No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG112840.15871017740967944No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA108330.1523668337361802No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC107420.15108691295061827No Hit
GTTGTAAGGCTTGCATAAAGGTTATTTGCTCAAAGCTATTCTCTGTAATT104350.14676893843229394No Hit
CTTAGGGACAACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGG104010.14629072627065542No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG98690.1388081124473703No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT98630.13872372206590466No Hit
GAATATAGGCTGTTGAACACCGTCAATGAAATGGAAGTCTGAATACATGA98620.13870965700232704No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT94890.1334633882878809No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG94330.1326757447275351No Hit
CTTCTAAGTGTGTGCATATTGCAGCGAACTTATTTGTTTCAATTTTCGGG93150.13101606722537787No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA92970.13076289608098102No Hit
GTTATATAGATTGTTCACGTTCGCATCATGCTCATCGAGTGTTTTTTGAT92070.1294970403589967No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT90620.12745760614024415No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG90550.12735915069520093No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTC90530.1273310205680457No Hit
ACAGTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG90090.12671215777063116No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGGTGT89640.126079229909639No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA87650.12328028225769587No Hit
ATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAATCGAGAAAACTAC86950.12229572780726364No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA86680.12191597109066832No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT86130.12114239259390011No Hit
ATTCTAGACACATGCACTATTGAAGGACTAGTCTATGGCAACCCTTCTTG85490.12024222852493349No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA83790.11785116771674087No Hit
GTACTGTTTAAGCCACCTTTTTCAGTCTGACATTGCACTACACAATTACC83740.11778084239885285No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA83350.1172323049193263No Hit
GCAATATGCACACACTTAGAAGTCTATATTCATCTCCACAACTCGAAGGA83230.11706352415639507No Hit
ACCTGGAACCTGGAACCTTTGATCTTGAAGGGCTATATGGAGCAATTGAG82550.11610709983311802No Hit
TTCTTAACCTATGTCGCATTTCTTCAATTAACCATCTTATCTCTTCAAAC82070.11543197678139305No Hit
GTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTC81910.11520693576415139No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC79640.11201416633203536No Hit
AATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGA79400.11167660480617288No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA79240.1114515637889312No Hit
ATATGGAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA79010.11112806732664633No Hit
GTGCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT78790.11081863592793904No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG78030.10974969109604119No Hit
GTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATC76640.10779464725875426No Hit
GTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCG76550.10766806168655584No Hit
CTATATAACAAGGTGAAGAGGGCACTGGGCTCCAATGCTATGGAAGATGG76250.10724610977922773No Hit
AGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGGAAGAGAATGGT75600.10633188064668349No Hit
GTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAA75470.10614903482017465No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT75170.10572708291284655No Hit
ACACAATTACCACCTTTTAAATCAGTCTTCAGGATTCTTCCATGGCTCCC75140.10568488772211374No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA74830.105248870751208No Hit
GAATATAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCTT74680.10503789479754395No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA73920.10396894996564608No Hit
TTCTACAGGAGTATGAGATGGCTGACTCAAAAGAGCGGTTTTTACCCTGT71400.10042455394408997No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA99300.0116.50412
GGTACTG106700.0108.493685
CAGGTAC111250.0103.937793
AGTAGAA43200.095.609411
GTACTGA123900.094.1344766
AGCAGGT143300.080.694091
AGGTACT154050.077.549834
ACTGATC182250.069.4852148
GCGAAAG12450.064.667092
GACTTAA2150.064.0665145
TTTGGAC132600.063.365483
TTTTTGG132900.062.757731
AGAAACA66400.062.2288974
AACACTG26900.061.1698655
CTGATCC193750.060.460649
CCGTATA4300.059.00862145
GACAGTA143100.058.8613747
TACTGAT216900.055.376687
GGACAGT154200.055.0943646
TTGGACA167500.054.011364
Produced by FastQC (version 0.11.5)